[1]侯智永.基于网络药理学及分子对接联合GEO芯片分析红花治疗冠心病的作用机制[J].大众科技,2023,25(1):86-91.
　Analysis of the Mechanism of Safflower in the Treatment of Coronary Heart Disease Based on Network Pharmacology and Molecular Docking Combined with GEO Chip[J].Popular Science & Technology,2023,25(1):86-91.
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基于网络药理学及分子对接联合GEO芯片分析红花治疗冠心病的作用机制()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
25
期数:
2023年1
页码:
86-91
栏目:
医药与卫生
出版日期:
2023-01-20

文章信息/Info

Title:
Analysis of the Mechanism of Safflower in the Treatment of Coronary Heart Disease Based on Network Pharmacology and Molecular Docking Combined with GEO Chip
作者:
侯智永　
（黑龙江省中医药科学院，黑龙江 哈尔滨 150000）
关键词:
红花; 冠心病; 网络药理学; GEO芯片; 分子对接
Keywords:
safflower coronary heart disease network pharmacology GEO chip molecular docking
文献标志码:
A
摘要:
运用网络药理学及分子对接联合GEO芯片分析的方法，分析红花治疗冠心病的分子靶点和作用机制。通过TCMSP平台筛选红花的有效成分和靶点；在GEO芯片数据库检索、分析、筛选芯片数据并提取冠心病的差异表达基因；将获得的药物靶点与疾病靶点取交集基因，使用STRING平台对关键靶点构建蛋白互作网络；利用Metascape数据库对关键靶点进行GO和KEGG富集分析，应用AutoDockTools软件进行分子对接，预测作用机制。共获得红花17个有效成分，对应218个作用靶点，药物靶点与疾病靶点交集基因22个；筛选到核心活性成分黄芩素、β-胡萝卜素、β-谷甾醇等，核心靶点VEGFA、MMP9、JUN等；GO和KEGG分析呈现生物过程199个，细胞组分17个，分子功能19个，富集通路涉及到IL-17信号通路、糖尿病并发症相关AGE-RAGE信号通路等；分子对接结果β-胡萝卜素、豆甾醇等与核心基因结合良好。文章初步揭示红花治疗冠心病的活性成分、作用靶点及信号通路，红花通过多成分、多靶点、多通路的方式发挥治疗冠心病的效用。
Abstract:
The molecular targets and mechanism of safflower in the treatment of coronary heart disease were analyzed by network pharmacology and molecular docking combined with GEO chip analysis.. The active components and targets of safflower were screened by TCMSP platform the microarray data were searched and analyzed in the GEO chip database and the differentially expressed genes of coronary heart disease were extracted the obtained drug targets and disease targets were intersected genes, and the STRING platform was used to build a protein interaction network for key targets GO and KEGG enrichment analysis was performed on key targets using Metascape database, and AutoDockTools software was used for molecular docking to predict the mechanism of action. A total of 17 active components of safflower were obtained, corresponding to 218 targets, and 22 genes were intersected between drug targets and disease targets the core active components such as baicalein, β-carotene, β-sitosterol, etc. MMP9, JUN, etc. were screened GO and KEGG analysis showed 199 biological processes, 17 cellular components, and 19 molecular functions. The enriched pathways involved IL-17 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, etc. molecular docking results β-carotene and stigmasterol were well combined with the core genes. The article preliminarily revealed the active components, action targets and signal pathways of safflower in the treatment of coronary heart disease. Safflower can play a role in the treatment of coronary heart disease through multiple components, multiple targets and multiple pathways.

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备注/Memo

备注/Memo:
【收稿日期】2022-10-29 【作者简介】侯智永（1996－），男，黑龙江省中医药科学院在读硕士研究生，研究方向为心血管疾病预防与治疗。
更新日期/Last Update: 2023-03-30