[1]张　杰　李军生　覃　懿　黄国霞　阎柳娟　马　纪.散射共振光谱法优化DNA去除溴化乙锭污染物[J].大众科技,2022,24(06):42-46.
　Optimization of DNA removal of ethidium bromide pollutants by scattering resonance spectroscopy[J].Popular Science & Technology,2022,24(06):42-46.
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散射共振光谱法优化DNA去除溴化乙锭污染物()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
24
期数:
2022年06
页码:
42-46
栏目:
出版日期:
2022-06-20

文章信息/Info

Title:
Optimization of DNA removal of ethidium bromide pollutants by scattering resonance spectroscopy
作者:
张　杰1; 2　李军生1; 2　覃　懿3　黄国霞1; 2　阎柳娟1; 2　马　纪1; 2　
（1.广西科技大学，广西 柳州 545006；2.广西糖资源绿色加工重点实验室，广西 柳州 545006；3.中国科技开发院广西分院，广西 南宁 530022）
关键词:
溴化乙锭; DNA; 去除
Keywords:
ethidium bromide DNA removal
文献标志码:
A
摘要:
文章利用紫外吸收光谱研究了DNA与溴化乙锭 (EtBr)的相互作用、散射共振光谱研究了二者的相互作用强度及影响因素，最后通过磁珠分离EtBr-DNA复合物以去除EtBr污染物。紫外可见吸收光谱结果表明DNA与EtBr的结合模式为嵌插结合。散射共振光谱和EtBr去除实验结果表明，CaCl2、SDS、CTAB、葡萄糖和尿素能够促进二者的结合，使DNA结合饱和值变大，提高EtBr的去除效率；NaCl抑制二者的结合，使DNA结合饱和值变小，降低EtBr的去除效率。研究为高效去除EtBr污染物提供新见解。
Abstract:
In this paper, the intercalation between DNA and ethidium bromide (EtBr) was studied by UV absorption spectroscopy, and the interaction intensity and influencing factors were studied by scattering resonance spectroscopy. Finally, the ETBR-DNA complex was separated by magnetic beads to remove EtBr contaminants. The UV-vis absorption spectroscopy results showed that the binding mode of DNA and EtBr was intercalation binding. The results of the resonance light scattering spectrometry and EtBr removal experiments showed that CaCl2, SDS, CTAB, glucose and urea can promote the combination of the two, increase the saturation value of DNA binding and improve the removal efficiency of EtBr NaCl inhibits the combination of the two, reduces the saturation value of DNA binding and reduces the removal efficiency of EtBr. This study provides new insights for the efficient removal of EtBr pollutants.

参考文献/References:

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备注/Memo

备注/Memo:
【收稿日期】2022-04-01 【基金项目】广西自然科学基金项目（2018GxNsFDA281030，2020GXNSFAAl59021）；国家自然科学基金项目（21966008）。 【作者简介】张杰（1993－），广西科技大学生物与化学工程学院研究生，研究方向为生物分子的化学修饰与功能研究。 【通信作者】李军生，广西科技大学生物与化学工程学院教授，硕士研究生导师，研究方向为生物分子的化学修饰与功能研究；覃懿，中国科技开发院广西分院工程师，研究方向为制药工程研究。
更新日期/Last Update: 2022-07-25