[1]吴广阳 唐 浪 詹 杰 赵 芬 黄 胜.基于网络药理学探讨竹节参防治肝癌的作用机制[J].大众科技,2022,24(02):114-118.
 Study on the Mechanism of Prevention and Treatment of Liver Cancer by Panax Japonicus Based on Network Pharmacology[J].Popular Science & Technology,2022,24(02):114-118.
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基于网络药理学探讨竹节参防治肝癌的 作用机制()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
24
期数:
2022年02
页码:
114-118
栏目:
医药与卫生
出版日期:
2022-02-20

文章信息/Info

Title:
Study on the Mechanism of Prevention and Treatment of Liver Cancer by Panax Japonicus Based on Network Pharmacology
作者:
吴广阳 唐 浪 詹 杰 赵 芬 黄 胜 
(湖北民族大学,湖北 恩施 445000)
关键词:
竹节参肝癌网络药理学靶点
Keywords:
Panax japonicus liver cancer network pharmacology target
文献标志码:
A
摘要:
目的:基于网络药理学探讨竹节参防治肝癌的作用机制。方法:利用TCMSP、Swiss Target Prediction数据库结合文本挖掘方式,提取竹节参的化合物成分、化合物靶点关系,利用OMIM和Gene Cards网站提取竹节参的疾病靶点,R语言V4.0.3绘制Venny图以获得交集靶点,相关信息导入Cytoscape V3.8.2以获得化合物-靶点-疾病网络;依据交集靶点,在STRING网站获得蛋白互作网络,R语言V4.0.3进行GO、KEGG富集分析。结果:在TCMSP、Swiss Target Prediction库中共获得15种有效化合物,包括皂苷、多糖、黄酮类等,对应靶点数259个,药物与疾病交集靶点57个,导入STRING网站发现连线较多的靶蛋白为AKT1、VEGFA、CASP3等,药物-有效化合物-靶点-疾病网络图表明,度值较高的化合物是竹节参皂苷Ⅳa、竹节参皂苷Ⅳa_qt、人参皂苷 Rg2_qt等;GO生物学过程富集分析发现:竹节参的关键靶蛋白主要通过调控内肽酶、蛋白丝氨酸/苏氨酸激酶、DNA结合转录激活活性RNA聚合酶II特异性等方式起到改善肝癌的作用;KEGG富集通路发现竹节参影响肝癌主要通过PI3K-AKT、卡波西肉瘤相关疱疹病毒感染、脂质和动脉粥样硬化等信号通路。结论:竹节参防治肝癌具有多靶点、多通路的特点,其治疗机制可能是通过调控AKT1、VEGFA、CASP3的活性有关。
Abstract:
Objective: To explore the mechanism of prevention and treatment of liver cancer of Panax japonicus based on network pharmacology. Methods: TCMSP, Swiss target prediction database combined with text mining were used to extract the compound components and compound target relationship of Panax japonicus, and OMIM and Gene Cards websites were used to extract the disease targets of Panax japonicus, R language V4.0.3 draw Venny diagram to obtain intersection targets, and import relevant information into Cytoscape V3.8.2 to obtain a compound target disease network, according to the intersection target, the protein interaction network was obtained at the string website, GO and KEGG enrichment analysis was performed in R language V4.0.3. Results: A total of 15 effective compounds, including saponins, polysaccharides and flavonoids, were obtained in tcmsp and Swiss target prediction libraries, with 259 corresponding target points and 57 drug disease intersection targets. It was found that the target proteins with more connections were AKT1, VEGFA and CASP3. The drug-effective compound-target-disease network diagram showed that the compounds with high degree value are Panax japonicus saponin Ⅳa and Panax japonicus saponin Ⅳa_qt, ginsenoside Rg2_qt, etc GO biological process enrichment analysis found that the key target proteins of Panax japonicus can improve liver cancer mainly by regulating endopeptidase, protein serine/threonine kinase, DNA binding transcriptional activation activity and RNA polymerase II specificity KEGG enrichment pathway found that bamboo ginseng affected liver cancer mainly through PI3K-AKT, Kaposi sarcoma associated herpesvirus infection, lipid and atherosclerosis. Conclusion: The prevention and treatment of liver cancer by Panax japonicus has the characteristics of multi-target and multi-channel. Its therapeutic mechanism may be related to regulating the activities of AKT1, VEGFA and CASP3.

参考文献/References:

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备注/Memo

备注/Memo:
【收稿日期】2021-11-04 【作者简介】吴广阳(1994-),男,湖北民族大学医学部在读硕士研究生,研究方向为民族医学。 【通信作者】黄胜(1987-),男,湖北民族大学研究生导师,研究方向为微生物来源天然产物的合成。
更新日期/Last Update: 2022-05-24