[1]聂雪玫 周雪芸 郑 霞.高效液相色谱法测定羧甲淀粉钠（浆状）中氯乙酸残留量[J].大众科技,2021,23(8):23-27.
　Determination of Residual Chloroacetic Acid in Sodium Carboxymethyl Starch(pulp) by High Performance Liquid Chromatography[J].Popular Science & Technology,2021,23(8):23-27.
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高效液相色谱法测定羧甲淀粉钠（浆状）中 氯乙酸残留量()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
23
期数:
2021年8
页码:
23-27
栏目:
出版日期:
2021-08-20

文章信息/Info

Title:
Determination of Residual Chloroacetic Acid in Sodium Carboxymethyl Starch(pulp) by High Performance Liquid Chromatography
作者:
聂雪玫 周雪芸 郑 霞
（广西南宁百会药业集团有限公司，广西 南宁 530032）
关键词:
羧甲淀粉钠; 高效液相色谱法; 氯乙酸
Keywords:
sodium carboxymethyl starch HPLC chloroacetic acid
文献标志码:
A
摘要:
目的：建立高效液相色谱法（HPLC）测定羧甲淀粉钠（浆状）中氯乙酸的残留量。方法：色谱柱为Titank C18（250 mm×4.6 mm，5 µm）；以0.2%磷酸溶液为流动相A，乙腈为流动相B，梯度洗脱；流速为1.0 mL/min，检测波长为214 nm，柱温为30℃，进样量为20 µL。结果：氯乙酸浓度在0.0056 mg/mL～0.2026 mg/mL范围内与峰面积呈良好的线性关系，r=1.0000（n=6），平均回收率为96.2%，RSD为1.14%（n=9）。结论：该方法操作简便、专属准确，可用于测定羧甲淀粉钠（浆状）中氯乙酸残留。
Abstract:
Objective: To establish a high performance liquid chromatography(HPLC) for the determination of chloroacetic acid residues in sodium carboxymethyl starch(pulp). Methods: The chromatographic column was Titank C18 (250mm×4.6mm, 5μm) the mobile phase a was 0.2% phosphoric acid solution, acetonitrile was the mobile phase B, and gradient elution was carried out the flow rate is 1.0mL/min, the detection wavelength is 214 nm, the column temperature is 30℃, and the sample injection volume is 20μL. Results: Chloroacetic acid showed a good linear relationship with peak area in the concentration range of 0.0056 mg/mL~0.2026 mg/mL, r=1.0000(n=6). The average recovery was 96.2%, RSD was 1.14%(n=9). Conclusion: The method is simple,specific and accurate, and can be used for the determination of chloroacetic acid residues in sodium carboxymethyl starch (pulp).

参考文献/References:

[1]　国家药典委员会. 中华人民共和国药典(四部)[M]. 北京: 中国医药科技出版社，2020. [2] 张锦梅，张苗苗，郑秀瑾，等. 离子色谱法测定羧甲基淀粉钠中羟基乙酸根、氯乙酸根、氯离子、磷酸根和硫酸根的含量[J]. 理化检验(化学分册)，2017(53): 611-614. [3] 赵霞，关浩月，伊利辉，等. 离子色谱法测定羧甲基淀粉钠中氯乙酸的残留[J]. 药物分析杂志2009，9(9): 1537-1539. [4] 顾林，李新旺，李静，等. 高效液相色谱法测定羧甲基淀粉钠中氯乙酸残留量[J]. 临床医药文献杂志，2017，40: 7726-7728. [5] GB/T28193－2011. 表面活性剂中氯乙酸(盐)残留量的测定[S]. 北京: 中华人民共和国质量监督检验检疫总局，2012.

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备注/Memo

备注/Memo:
【收稿日期】2021-06-09 【作者简介】聂雪玫（1986－），女，广西南宁百会药业集团有限公司研发工程师，研究生，从事药物分析工作。
更新日期/Last Update: 2021-11-09