[1]陈松林 黄 燕 冯金鹏 杨 磊.慢性乙型肝炎患者外周血中B7-H3 变化趋势分析[J].大众科技,2019,21(01):20-22,29.
　Analysis of B7-H3 Trend in Peripheral Blood of Patients with Chronic Hepatitis B[J].Popular Science & Technology,2019,21(01):20-22,29.
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慢性乙型肝炎患者外周血中B7-H3 变化趋势分析()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
21
期数:
2019年01
页码:
20-22,29
栏目:
医药与卫生
出版日期:
2019-01-20

文章信息/Info

Title:
Analysis of B7-H3 Trend in Peripheral Blood of Patients with Chronic Hepatitis B
作者:
陈松林 黄 燕 冯金鹏 杨 磊
（广西中医药大学第一附属医院，广西 南宁 530021）
关键词:
乙型肝炎; B7-H3; 重新分布; HBV DNA
Keywords:
Hepatitis B;  B7-H3;  redistribution;  HBV DNA
文献标志码:
A
摘要:
目的：探讨慢性乙型肝炎患者外周血中B7-H3 变化趋势。方法：收集广西中医药大学第一附属医院2015 年5月至2016 年6 月肝病科患者血清样本共47 例，根据其乙肝两对半结果而分为4 组，即大三阳组、小三阳组、慢性乙型肝炎组、肝纤维化组，并从广西中医药大学第一附属医院体检中心随机收取10 份HBsAg 阴性健康人血清样本作为对照组。用ELISA 方法检测其血清样本中B7-H3 含量，并两两比较各组之间的差异；用RT-PCR 的方法检测患者组样本血清中HBV DNA 拷贝数，并分析其与B7-H3 的相关性。结果：对照组血清样本中B7-H3 含量为（7.36±0.36）ng/mL，高于另外4 组患者组，差异有统计学意义（P＜0.05）；患者组4 组B7-H3 含量均为（2.12～2.81）ng/mL 之间，4 组间两两比较均无统计学意义（P＞0.05）；4 组患者组HBV DNA 检测结果显示，大三阳组HBV DNA 拷贝数达到（602.2±193.2）×105/mL，与肝纤维化组相比无统计学差异（P＞0.05）；大三阳组、肝纤维化组患者HBV DNA 拷贝数与小三阳组、慢性HBV 感染组相比，其拷贝数明显升高，差异有统计学意义（P＜0.05）；所有患者组样本中B7-H3 含量与HBV DNA 拷贝数相关性分析结果显示，二者无明显相关性（P＞0.05）。结论：乙型肝炎患者外周血中B7-H3 含量与正常人相比有所下降，可能与B7-H3 在患者体内重新分布有关，与乙型肝炎病毒的活跃程度无明显相关性。
Abstract:
Objective: To investigate the changes of B7-H3 in peripheral blood of patients with chronic hepatitis B. Methods: A total of 47 serum samples from patients with hepatic diseases from May 2015 to June 2016 were collected. According to the results of detection of hepatitis B, they were divided into 4 groups, namely, Da-san-yang group, Xiao-san-yang group and chronic hepatitis B group, and liver fibrosis group, respectively. Ten HBsAg-negative healthy human serum samples collected from the First Affiliated Hospital of Guangxi University of Chinese Medicine were as the control group. The B7-H3 content in the serum samples were measured by ELISA, and the differences between each two groups were compared by SPSS. Results: The B7-H3 content in the serum samples of the control group was (7.36±0.36) ng/mL, which was higher than that of the other 4 groups (P＜0.05). The B7-H3 contents of the patients in the 4 groups were the range of 2.12ng/mL and 2.81ng/ml, and there was no significant difference between each two groups (P＞0.05). The results of RT-PCR assay of the four groups showed that the copies of HBV DNA in the Da-san-yang group reached (602.2±193.2)×105/mL, which was not significantly different from the liver fibrosis group (P＞0.05). The copies of HBV DNA in the patients of Da-san-yang group and liver fibrosis group were significantly higher than that in Xiaosanyang group and chronic HBV infection group, the difference was statistically significant (P＜0.05). The result of correlation analysis between B7-H3 content and HBV DNA copy number in all patient samples showed that there was no significant correlation between the two factors (P＞0.05).Conclusion: The content of B7-H3 in peripheral blood of patients with hepatitis B is lower than that of normal people, which seems to be related to the redistribution of B7-H3 in the body of patient, and there were no significant correlation between the content of B7-H3 and the activity of hepatitis B virus.

参考文献/References:

【参考文献】 [1] Fang,C,Li S,Xu R,et al.,Soluble B7-H3 (sB7-H3) is over- expressed in the serum of type 1 diabetes patients[J]. Diabetes Research and Clinical Practice,2018,143: 332-336. [2] Guo,G,Cao D,Xu H,et al.The characteristic expression of B7-H3 and B7-H4 in liver biopsies from patients with HBV-related acute-on-chronic liver failure[J].Pathology International,2012,62(10):665-674. [3] Hwang J Y,Jeong J M,Kwon M G,et al.Olive flounder CD276 (B7-H3) a coinhibitory molecule for T cells: Responses during viral hemorrhagic septicemia virus (VHSV) stimulation[J].Fish & Shellfish Immunology,2018, 73:228-233. [4] Luan,Y,Ju J,Luo L,et al.Potential role of soluble B7-H3 in liver immunopathogenesis during chronic HBV infection [J].Journal of Viral Hepatitis,2012,19(1):23-31. [5] Scilla K A,Zandberg D P,Bentzen S M,et al.Case-control study of PD-1,PD-L1 and B7-H3 expression in lung cancer patients with and without human immunodeficiency virus (HIV) infection [J].Lung Cancer,2018,123: 87-90. [6] Seaman S,Zhu Z,Saha S,et al.,Eradication of tumors through simultaneous ablation of CD276/B7-H3-positive tumor cells and tumor vasculature [J].Cancer Cell,2017,31(4):501-515. [7] He C S,Liu Y C,Xu Z P,et al.Astragaloside IV enhances cisplatin chemosensitivity in non-small cell lung cancer cells through inhibition of B7-H3[J].Cellular Physiology and Biochemistry,2016,40(5):1221-1229. [8] Hwang JY,Jeong JM,Kwon MG,et al.Olive flounder CD276 (B7-H3) a coinhibitory molecule for T cells:Responses during viral hemorrhagic septicemia virus (VHSV) stimu lation[J].Fish Shellfish Immunol,2018,73: 228-233. [9] Kim,G E,Kim N I,Park M H,et al.B7-H3 and B7-H4 expression in phyllodes tumors of the breast detected by RNA in situ hybridization and immunohistochemistry: Association with clinicopathological features and T-cell infiltration [J].Tumour Biology,2018,40(11):1010428318815032. [10] Luan,Y,Ju J,Luo L,et al.Potential role of soluble B7-H3 in liver immunopathogenesis during chronic HBV infection [J].Journal of Viral Hepatitis,2012,19(1):23-31. [11] Mao L,Fan T F,Wu L,et al.,Selective blockade of B7-H3 enhances antitumour immune activity by reducing immature myeloid cells in head and neck squamous cell carcinoma [J].Journal of Cellular and Molecular Medicine,2017,21(9): 2199-2210. [12] Pizon M,Schott D S,Pachmann U,et al.B7-H3 on circulating epithelial tumor cells correlates with the proliferation marker, Ki-67,and may be associated with the aggressiveness of tumors in breast cancer patients [J].International Journal of Oncology,2018,53(5):2289-2299. [13] Scilla,K A,Zandberg D P,Bentzen S M,et al.,Case-control study of PD-1,PD-L1 and B7-H3 expression in lung cancer patients with and without human immunodeficiency virus (HIV) infection [J].Lung Cancer,2018,123: 87-90. [14] Mao Y,Sun J,Wang WP,et al.Clinical significance of costimulatory molecule B7-H3 expression on CD3(＋) T cells in colorectal carcinoma [J],Chinese Medical Journal, 2013,126(16): 3035-3038. [15] Wilson,K E,Bachawal S V,Willmann J K.Intraoperative resection guidance with photoacoustic and fluorescence molecular imaging using an anti-B7-H3 antibody-indocyanine green dual contrast agent[J].Clinical Cancer Research,2018, 24(15):3572-3582.[16] Xu F,Yi J,Wang F,et al.,Involvement of soluble B7-H3 in combination with the serum inflammatory cytokines interleukin-17,-8 and -6 in the diagnosis of hepatocellular carcinoma [J].Oncology Letters,2017,14(6):8138-8143. [17] Xu J C,Li M,Ma,et al.The Expression of B7-H3 in circulating CD4＋CD25 high T cells,circulating CD14＋ monocytes and plasma during hepatitis B virus infection progression[J].Clinical Laboratory,2017,63(3):617-623. [18] Zhili X,Ling W,Jianhua T,et al.High expression of B7-H3 and CD163 in cancer tissues indicates malignant clinicopathological status and poor prognosis of patients with urothelial cell carcinoma of the bladder[J].Oncology Letters,2018,15(5): 6519-6526. [19] 闫新星,张巍,王晶,等.沉默B7-H3 基因抑制人恶性血液病裸鼠移植瘤的体内成瘤和转移[J].北京大学学报(医学版), 2017,49(2):286-294. [20] 张巍,王晶,王艳芳,等.共刺激分子B7-H1、B7-H3 和B7-H4在血液系统恶性肿瘤细胞株中的表达水平和分布特征[J].中国实验血液学杂志,2016,24(5):1539-1546.

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备注/Memo

备注/Memo:
【收稿日期】2018-11-06 【基金项目】广西壮族自治区教育厅“广西高校科研项目”（YB2014170）；广西中医药大学第一附属医院青年基金（2016QN015）。 【作者简介】陈松林，广西中医药大学第一附属医院检验科副主任技师，临床免疫学硕士，从事临床免疫学诊断及分子生物学诊断工作。 【通信作者】杨磊，广西中医药大学第一附属医院检验科技师，临床检验诊断学硕士，从事分子诊断学工作及研究。
更新日期/Last Update: 2019-02-21