[1]李?祎,毛?彦,黄?丽,等.六堡茶多糖对高脂血症大鼠结肠微生态的影响[J].大众科技,2023,25(2):99-104.
　Effect of Liupao Tea Polysaccharide on Colonic Microecology in Hyperlipidemia Rats[J].Popular Science & Technology,2023,25(2):99-104.
点击复制

六堡茶多糖对高脂血症大鼠结肠微生态的影响()
分享到：

《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
25
期数:
2023年2
页码:
99-104
栏目:
医药与卫生
出版日期:
2023-02-20

文章信息/Info

Title:
Effect of Liupao Tea Polysaccharide on Colonic Microecology in Hyperlipidemia Rats
作者:
李?祎; 毛?彦 ;  黄?丽; ?夏?宁; ?滕建文 ; 韦保耀
（广西大学轻工与食品工程学院，广西 南宁 530004）
关键词:
Liupao tea;  Liupao tea polysaccharide;  hyperlipidemia;  hypolipidemic;  intestinal flora
Keywords:
Liupao tea;  Liupao tea polysaccharide;  hyperlipidemia;  hypolipidemic;  intestinal flora
文献标志码:
A
摘要:
为确定六堡茶多糖对肠道的调节功能，本研究对高脂血症大鼠连续4周灌胃100mg/kg·bw、300mg/kg·bw和500 mg/kg·bw 六堡茶多糖，结果表明：六堡茶多糖中高剂量组大鼠结肠内容物质量、丙酸、丁酸和总短链脂肪酸含量显著升高。高通量Illumina Hiseq测序结果及物种差异、相关分析表明：六堡茶多糖能显著降低厚壁菌门与拟杆菌门的比值，提升大鼠肠道菌群中的普氏菌属、瘤胃球菌属、拟杆菌属、拟普氏菌属、经黏液真杆菌属、乳杆菌属等有益菌属的丰度，降低毛螺菌属、苏黎世杆菌属、真杆菌属等属的丰度，其中瘤胃球菌属，经黏液真杆菌属等丰度的增加与大鼠的血脂水平改善正相关。说明六堡茶多糖可以通过上调有益菌属的丰度，改善大鼠肠道菌群结构，促进结肠的短链脂肪酸的含量增加，达到调节血脂作用。
Abstract:
?n order to determine the regulatory function of Liupao tea polysaccharide on intestinal tract, hyperlipidemia rats were intragastric administrated with Liupao tea polysaccharide of 100, 300 and 500 mg/kg?bw for 4 weeks. The results showed that the mass of colon contents, propionic acid, butyric acid and total short chain fatty acid content of rats in the middle and high dose group of Liupao tea polysaccharide were significantly increased. The high-throughput Illumina Hiseq sequencing results, species differences and correlation analysis showed that Liupao tea polysaccharide can significantly reduce the ratio of firmicutes to bacteroidetes, increase the abundance of beneficial bacteria such as prevotella,ruminococcus, bacteroidetes, alloprevotella, blautia, lactobacillus and other beneficial bacteria in rat intestinal flora, and decrease the abundance of lachnospiraceae, turicibacter, eubacterium and other genera, among them, the increase of abundance of rumococcus and blautia is positively correlated with the improvement of blood lipid level in rats. It shows that Liupao tea polysaccharide can regulate blood lipids by up-regulating the abundance of beneficial bacteria, improving the structure of intestinal flora in rats, and promoting the content of short-chain fatty acids in the colon.

参考文献/References:

[1]　B?KHED F, CRAWFORD P A. Coordinated regulation of the metabolome and lipidome at the host-microbial interface[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2010, 1801(3): 240-245.
[2] VOREADES N, KOZIL A, WEIR T L. Diet and the development of the human intestinal microbiome[J]. Frontiers in Microbiology, 2014, 5: 494.
[3] WEERSMA R K, ZHERNAKOVA A, FU J. Interaction between drugs and the gut microbiome[J]. Gut, 2020, 69(8): 1510-1519.
[4] FLANDROY L, POUTAHIDIS T, BERG G, et al. The impact of human activities and lifestyles on the interlinked microbiota and health of humans and of ecosystems[J]. Science of the Total Environment, 2018, 627: 1018-1038.
[5] CONLON M A, BIRD A R. The impact of diet and lifestyle on gut microbiota and human health[J]. Nutrients, 2014, 7(1): 17-44.
[6] 刘荣瑜，王昊，张子依，等. 多糖与肠道菌群相互作用的研究进展[J]. 食品科学，2022，43(5): 363-373.
[7] NORDGAARD I, MORTENSEN P B. Digestive processes in the human colon[J]. Nutrition, 1995, 11(1): 37-45.
[8] 石荣强，温立香，曾玉凤，等. 六堡茶品质研究进展[J]. 中国茶叶加工，2020(2): 43-47.
[9] MAO Y, WEI B, TENG J, et al. Polysaccharides from Chinese Liupao dark tea and their protective effect against hyperlipidemia[J]. International Journal of Food Science and Technology, 2018, 53(3): 599-607.
[10] 尹凯歌，温宝江，牛亚楠，等. 溃疡性结肠炎小鼠肠道黏膜和盲肠内容物菌群差异比较[J]. 中国微生态学杂志，2022，34(5): 511-517.
[11] 赵文静，刘书云，丁金梅，等. 小鼠不同肠道段内容物和粪便中微生物的宏基因组测序和比较分析[J]. 上海交通大学学报(农业科学版)，2016，34(3): 15-21.
[12] 李胜方，刘先利，滕雷，等. 多糖用作结肠靶向给药载体的研究进展[J]. 黄石理工学院学报，2008(1): 35-39.
[13] 姜金池. 益生菌对高胆固醇血症的缓解作用及机制研究[D]. 无锡: 江南大学，2021.
[14] 廖培龙，李欢，陈剑，等. 大豆益生元与肠道菌群相互作用及其对健康影响的研究进展[J]. 食品科学，2016，37(7): 1-17.
[15] ABDEL-LATIF H M R, ABDEL-TAWWAB M, DAWOOD M A O, et al. Benefits of dietary butyric acid, sodium butyrate, and their protected forms in aquafeeds: A review[J]. Reviews in Fisheries Science and Aquaculture, 2020, 28(4): 421-448.
[16] 宋艳，李岳飞，王智鼎，等. 嗜酸乳杆菌高密度培养工艺条件的优化及其评价[J]. 吉林大学学报(医学版)，2013，39(5): 1036-1040.
[17] 任多多，李珊珊，薄盼盼，等. 西洋参多糖对大鼠肠道菌群的影响 [J]. 食品科技，2022，47(6): 211-217.
[18] NARDELLI C, GRANATA I, D’ARGENIO V, et al. Characterization of the duodenal mucosal microbiome in obese adult subjects by 16S rRNA sequencing[J]. Microorganisms, 2020, 8(4): 485.
[19] VEBO H C, KARLSSON M K, AVERSHINA E, et al. Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome[J]. Journal of Microbiological Methods, 2016, 129: 78-80.
[20] 刘孟洋. 绣球菌多糖酵解特征体系及其对小鼠肠道微生态系统的影响[D]. 太原: 山西农业大学，2019.
[21] 丁郁，朱振军，黄锐，等. 蛹虫草多糖在制备调节高脂饮食引起的肠道菌群和代谢物紊乱产品中的应用: 中国，114224908A [P]. 2022-03-25.
[22] REY F E, FAITH J J, BAIN J, et al. Dissecting the in vivo metabolic potential of two human gut acetogens[J]. Journal of Biological Chemistry, 2010, 285(29): 22082-22090.
[23] GOPHNA U, KONIKOFF T, NIELSEN H B. Oscillospira and related bacteria: From metagenomic species to metabolic features[J]. Environmental Microbiology, 2017, 19(3): 835-841.
[24] VITAL M, HOWE A C, TIEDJE J M. Revealing the bacterial butyrate synthesis pathways by analyzing (meta) genomic data[J]. mBio, 2014, 5(2): e00889-e00914.
[25] LIU X, MAO B, GU J, et al. Blautia-a new functional genus with potential probiotic properties[J]. Gut Microbes, 2021, 13(1): 1875796.
[26] ZHAO L, ZHANG Q, MA W, et al. A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota[J]. Food and function, 2017, 8(12): 4644-4656.?/div>

备注/Memo

备注/Memo:
【收稿日期】2022-07-18
【基金项目】国家自然科学基金项目（32160571）；广西重点研发计划（AB21220068）。
【作者简介】李祎（1998－），女，广西大学轻工与食品工程学院在读硕士研究生，研究方向为食品加工与安全。
【通信作者】黄丽（1978－），女，广西大学轻工与食品工程学院副教授，研究方向为食品微生物与安全。
更新日期/Last Update: 2023-04-25