[1]林勃勃 韦庆敏 刘 发 罗志辉.竞争法制备喹乙醇快速检测试纸条及其在鲫鱼检测中应用[J].大众科技,2022,24(12):34-38.
　Preparation of Rapid Detection Strip of Olaquindox for Competitive Method and Its Application in the Detection of Crucian Carp[J].Popular Science & Technology,2022,24(12):34-38.
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竞争法制备喹乙醇快速检测试纸条及其在 鲫鱼检测中应用()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
24
期数:
2022年12
页码:
34-38
栏目:
出版日期:
2022-12-20

文章信息/Info

Title:
Preparation of Rapid Detection Strip of Olaquindox for Competitive Method and Its Application in the Detection of Crucian Carp
作者:
林勃勃1 韦庆敏1 刘 发1 罗志辉1; 2; 3　
（1.玉林师范学院，广西 玉林 537000； 2.广西农产资源化学与生物技术重点实验室，广西 玉林 537000； 3.广西高校桂东南特色农产资源高效利用重点实验室，广西 玉林 537000）
关键词:
喹乙醇; 胶体金; 竞争法; 快速检测; 试纸条
Keywords:
olaquindox colloidal gold competitive method rapid detection test strip
文献标志码:
A
摘要:
目的：在胶体金免疫层析技术的基础上采用竞争法制备喹乙醇快速检测试纸条。方法：采用一锅法合成显色用的胶体金，调控柠檬酸钠的量，获得不同最大吸收波长的胶体金。通过对胶体金吸收波长、碳酸钾添加量等条件的优化，获得胶体金的最大吸收波长为522 nm、碳酸钾（0.2 mmol/L）添加量为15 μL的最佳条件，在此最优条件下制备试纸条，考察试纸条的方法学参数，并将制备好的试纸条检测实际样品。结果：随着喹乙醇浓度的减小（5.3×10-1 mg/mL，5.3×10-7 mg/mL），试纸条的控制线C线一直显色，而检测线T线逐渐清晰、显色，表明试纸条是准确的，检出限为5.3×10-7 mg/mL，所有的检测在5～8分钟内能完成，并成功用于鲫鱼实际样品的检测。结论：该试纸条适用于对肉类中的喹乙醇残留进行高效灵敏的检测，有利于保障人们的食品安全。
Abstract:
Objective: On the basis of colloidal gold immunochromatography technology, competitive method was used to prepare rapid test strips for olaquindox. Methods: The colloidal gold for color development was synthesized by one pot method, and the amount of sodium citrate was adjusted to obtain colloidal gold with different maximum absorption wavelengths. By optimizing the absorption wavelength of colloidal gold and the addition amount of potassium carbonate, the maximum absorption wavelength of colloidal gold was 522 nm and the addition amount of potassium carbonate (0.2 mmol/L) was 15 μL, prepare the test strip under this optimal condition, investigate the methodological parameters of the test strip, and test the actual sample with the prepared test strip. Results: With the decrease of olaquindox concentration (5.3×10-1 mg/mL, 5.3×10-7 mg/mL), the control line C of the test strip showed color all the time, while the detection line T was gradually clear and colored, indicating that the test strip was accurate, and the detection limit was 5.3×10-7 mg/mL, all tests could be completed within 5 to 8 minutes, and successfully used for the detection of actual samples of crucian carp. Conclusion: The test strip is suitable for the highly efficient and sensitive detection of olaquindox residues in meat, which is conducive to ensuring the safety and health of human food.

参考文献/References:

[1]　BARBER R S, BRAUDE R, HOSKING Z D, et al. Olaquindox as performance-promoting feed additive for growing pigs[J]. Animal Feed Science and Technology, 1979, 4(2), 117-123. [2]　HAN J, JIANG D, CHEN T, et al. Simultaneous determination of olaquindox, oxytetracycline and chlorotetracycline in feeds by high performance liquid chromatography with ultraviolet and fluorescence detection adopting online synchronous derivation and separation[J]. Journal of Chromatography B: Biomedical Sciences and Applications, 2020, 1152: 122253. [3]　GE L, GAO Y Q, HAN Z, et al. Administration of olaquindox impairs spermatogenesis and sperm quality by increasing oxidative stress and early apoptosis in mice[J]. Ecotoxicology and Environmental Safety, 2022, 234: 113396. [4]　LI D, ZHANG Y, PEI X, et al. Molecular mechanism of olaquindox-induced hepatotoxicity and the hepatic protective role of curcumin[J]. Food and Chemical Toxicology, 2020, 145: 111727. [5]　KHAN N A, AHMED S, FAROOQI I H, et al. Occurrence, sources and conventional treatment techniques for various antibiotics present in hospital wastewaters: A critical review[J]. Trends in Analytical Chemistry, 2020, 129: 115921. [6]　BORRAS S, COMPANYO R, GRANADOS M, et al. Analysis of antimicrobial agents in animal feed[J]. Trends in Analytical Chemistry, 2011, 30(7): 1042-1064. [7]　殷居易，倪梅林，寿成杰，等. 鸡肉中喹乙醇、卡巴多及其代谢物的残留检测[J]. 中国兽药杂志，2006，40(1): 11-15. [8]　HE S, WANG Q, Li S, et al. Antibiotic growth promoter olaquindox increases pathogen susceptibility in fish by inducing gut microbiota dysbiosis[J]. Science China(Life sciences), 2017, 60(11): 1260-1270. [9] 曾静，朱宽正，王鹏，等. 高效液相色谱-串联质谱法测定水产品中的喹乙醇[J]. 中国食品卫生杂志，2006，18(5): 423-425. [10] 邹淼，蒋莹，冯静，等. 超高效液相色谱串联质谱法测定鸡蛋中喹乙醇及卡巴氧代谢物[J]. 食品安全质量检测学报，2020，11 (19): 6942-6946. [11] 任龙梅. 超高效液相色谱-串联质谱法测定畜肉中喹乙醇和代谢物残留及其冷冻储藏稳定性研究[J]. 食品安全质量检测学报，2021，12 (11): 4559-4565. [12] MIAO X, XU L, LI H, et al. Determination of olaquindox, carbadox and cyadox in animal feeds by ultra-performance liquid chromatography tandem mass spectrometry[J]. Food Additives and Contaminants, 2018, 35(7): 1257-1265. [13] ZHANG H, QU W, DING C, et al. Tissue depletion of olaquindox and its six metabolites in pigs and broilers: Identification of a suitable marker residue[J]. Frontiers in Veterinary Science, 2021, 8: 638358. [14] HASSANTABAR F, ZORRIEHZAHRA M J, Firouzbakhsh F, et al. Development and evaluation of colloidal gold immunochromatography test strip for rapid diagnosis of nervous necrosis virus in golden grey mullet (Chelon aurata) [J]. Journal of Fish Diseases, 2021, 44 (6): 783-791. [15] CHENG L, SHEN J, WANG Z, et al. A sensitive and specific ELISA for determining a residue marker of three quinoxaline antibiotics in swine liver[J]. Analytical and Bioanalytical Chemistry, 2013, 405(8): 2653-2659. [16] WANG Q, HOU M L, LIU L P, et al. New method for ultra-sensitive P24 antigen assay based on near-infrared fluorescent microsphere immunochromatography[J]. Biomedical and Environmental Science, 2020, 33(3): 174-182. [17] WU Y, ZHANG J, YANG X, et al. Early diagnosis of ocult blood of colorectal cancer based on nano-colloidal gold sandwich immunochromatography[J]. Journal of Biomedical Nanotechnology, 2021, 17(8): 1525-1534. [18] LIU D, WANG A, ZHOU J, et al. A label-free electrochemical immunosensor based on AuNPs/GO-PEI-Ag-Nf for olaquindox detection in feedstuffs[J]. Microchemical Journal, 2022, 177: 107287. [19] STARA V, KOPANICA M. Determination of some quinoxaline-N-dioxide derivatives by adsorptive stripping voltammetry[J]. Analytica Chimica Acta, 1986, 186: 21-30. [20] 谢小华，周德山，宋向明，等. 高效液相色谱法测定水产品中喹乙醇残留量[J]. 理化检验(化学分册)，2011，47(1): 102-103. [21] KUMAR P, SARKAR N, SINGGH A, et al. Nanopaper biosensors at point of care[J]. Bioconjugate Chemistry, 2022, 33(6): 1114-1130. [22] SENATORRALBA A, ALVAREZDIDK R, PAROLO C, et al. Toward next generation lateral flow assays: Integration of nanomaterials[J]. Chemical Reviews, 2022, 112(18): 14881-14910. [23] LI J, WU T, WANG C, et al. Nanogapped Fe3O4@Au surface-enhanced raman scattering tags for the multiplex detection of bacteria on an immunochromatographic strip[J]. ACS Applied Nano Materials, 2022, 5(9): 12679-12689. [24] FAHEEM A, QIN Y, NAN W, et al. Advances in the Immunoassays for Detection of Bacillus thuringiensis Crystalline Toxins[J]. Journal of Agricultural and Food Chemistry, 2021, 69(36): 10407-10418. [25] SU L, WANG L, XU J, et al. Competitive lateral flow immunoassay relying on Au–SiO2 janus nanoparticles with an asymmetric structure and function for furazolidone residue monitoring[J]. Journal of Agricultural and Food Chemistry, 2021, 69(1): 511-519. [26] MILLS C, DILLON M J, KULABJUSAN P K, et al. Multiplex lateral flow assay and the sample preparation method for the simultaneous detection of three marine toxins[J]. Environmental Science and Technology, 2022, 56(17): 12210-12217. [27] WU S, LIU L, DUAN N, et al. Aptamer-based lateral flow test strip for rapid detection of zearalenone in corn samples[J]. Journal of Agricultural and Food Chemistry, 2018, 66(8): 1949-1954. [28] FRENS G. Controlled nucleation for regulation of particle-size in monodisperse gold suspensions[J]. Nature Physical Science, 1973, 241(105): 20-22. [29] WU Y, ZHOU Y, LENG Y, et al. Emerging design strategies for constructing multiplex lateral flow test strip sensors[J]. Biosensors and Bioelectronics, 2020, 157: 112168. [30] CHENG Y, GE W, KUANG H, et al. Gold-based immunochromatographic strip for rapid ketoconazole detection[J]. Microchemical Journal, 2022, 174: 107083.

备注/Memo

备注/Memo:
【收稿日期】2022-09-29 【基金项目】国家自然科学基金（22064018）；广西自然科学基金（2019JJA120031、2019GXNSFAA245026）；玉林市科技攻关项目（201934012）；学校高层次人才科研启动项目（G2020ZK04、G2021ZK15）；广西教育厅人才项目（2018-18）；大学生创新项目（201910606143）。 【作者简介】林勃勃（1998－），女，玉林师范学院学生，从事食品快速方法研究。 【通信作者】罗志辉，男，玉林师范学院教授，从事纳米材料的制备及其分析应用、食品安全检测研究。
更新日期/Last Update: 2023-03-21