[1]徐倩岚　黄　萍　王晴楠　庞　博　王万山　张嘉宁.频谱水对KM小鼠抗氧化作用的研究[J].大众科技,2021,23(12):38-41.
　Antioxidant Effect of Spectrum Water on KM Mice[J].Popular Science & Technology,2021,23(12):38-41.
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频谱水对KM小鼠抗氧化作用的研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
23
期数:
2021年12
页码:
38-41
栏目:
医药与卫生
出版日期:
2021-12-20

文章信息/Info

Title:
Antioxidant Effect of Spectrum Water on KM Mice
作者:
徐倩岚1　黄　萍1　王晴楠1　庞　博2　王万山1　张嘉宁3　
（1.南方医科大学实验动物管理中心，广东 广州 510515； 2.南方医科大学第二临床学院，广东 广州 510515； 3.广州南方医大实验动物科技发展有限公司，广东 广州 510515）
关键词:
小鼠; 抗氧化功能; 频谱水
Keywords:
mice antioxidant effect spectrum water
文献标志码:
A
摘要:
目的：探讨频谱水对SPF级KM小鼠抗氧化作用。方法：60只SPF级KM小鼠（♀），分A、B和C组，每组20只KM小鼠。A组动物每天泡浴频谱水45 分钟同时自由饮用频谱水；B组动物每天泡浴频谱水45分钟同时自由饮用自来水；C组动物每天泡浴自来水45 分钟同时自由饮用自来水，实验时间为40天。实验结束后动物采血，进行MDA含量、SOD活性及GSH-Px活性的测定。结果： MDA含量A组与C组比较，差异极显著（P＜0.01）、B组与C组比较，差异有显著性意义（P＜0.05）；SOD活性A组与B组比较，差异极显著（P＜0.01），B组与C组比较，差异有显著性意义（P＜0.05），A组与C组比较，差异极显著（P＜0.01）；GSH-Px活性A组与C组比较，差异极显著（P＜0.01）、B组与C组比较，差异有显著性意义（P＜0.05），A组与B组比较，差异极显著（P＜0.01）。结论：KM小鼠频谱水泡浴和饮用频谱水或者单纯频谱水泡浴均能提高超氧化物歧化酶和谷胱甘肽过氧化物酶活性，降低丙二醛含量，尤其是频谱水泡浴和饮用效果最为明显。
Abstract:
Objective: To investigate the antioxidant effect of spectrum water on SPF KM mice. Methods: A total of 60 SPF KM male mouse were divided into groups A, B and C, with 20 KM mice in each group. The animals in group A bathed in spectrum water for 45 minutes every day and drank spectrum water freely at the same time the animals in group B bathed in spectrum water for 45 minutes every day and drank tap water freely at the same time the animals in group C bathed in tap water for 45 minutes every day and drank tap water freely at the same time for 40 days. After the experiment, the animals took blood for the determination of MDA content, SOD activity and GSH PX activity determination. Results: There was significant difference in the content of MDA between group A and group C (P<0.01), and there was significant difference between group B and group C (P<0.05) there was significant difference in SOD activity between group A and group B (P<0.01), there was significant difference between group B and group C (P<0.05), and there was significant difference between group A and group C (P<0.01) there was significant difference in GSH-PX activity between group A and group C (P<0.01), there was significant difference between group B and group C (P<0.05), and there was significant difference between group A and group B (P<0.01). Conclusion: Spectrum bubble bath and drinking spectrum water or spectrum bubble bath alone can increase the activities of superoxide dismutase and glutathione peroxidase and reduce the content of malondialdehyde in KM mice. In particular, the spectrum bubble bath and drinking effect are the most obvious.

参考文献/References:

[1]　袁进，王玉珏，顾为望. 频谱水对SPF级KM小鼠的抗疲劳作用[J]. 中国比较医学杂志，2009，19(5): 47-49. [2]　张嘉宁，张建明，黄琼，等. 频谱水泡浴加饮用对小鼠免疫功能的影响[J]. 实验动物与比较医学，2009，29(6): 397-398，401. [3]　刘育英，赵秀梅，刘凤英，等. 频谱水对大鼠胃肠电位及蠕动功能的影响[J]. 中国临床康复，2004，8(3): 479-481. [4]　de Zwart L L, Meerman J H, Commandeur J N, et al. Biomarkers of free radical damage applications in experimental animals and in humans[J]. Free Radical Biology and Medicine, 1999, 26(1-2): 202-226. [5]　Marnett L J. Chemistry and biology of DNA damage by malondialdehyde[J]. IARC Scientific Publications, 1999(150): 17-27. [6]　Famurewa A C, Maduagwuna E K, Folawiyo A M, et al. Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-?/caspase-3 signaling pathway in Wistar rats[J]. Journal of Food Biochemistry, 2020, 44(1): e13100. [7]　陈永芬，申琳，鱼泳泳，等. 低聚原花青素方剂对小鼠肝脏、血清中相关抗氧化酶活性及MDA、活性氧含量的影响[J]. 食品科学，2004(S1): 164-167. [8]　Masalkar P D, Abhang S A. Oxidative stress and antioxidant status in patients with alcoholic liver disease[J]. Clinica Chimica Acta, 2005, 355(1-2): 61-65.

备注/Memo

备注/Memo:
【收稿日期】2021-10-08 【作者简介】徐倩岚（1991－），女，南方医科大学实验动物管理中心助理实验师，从事动物实验研究工作。
更新日期/Last Update: 2022-04-07