[1]马　南　何志义.关于红霉素通过调控RBBP7减轻COPD小鼠糖皮质激素抵抗的研究[J].大众科技,2021,23(11):87-90.
　Study on Erythromycin Reducing Glucocorticoid Resistance in COPD Mice by Regulating RBBP7[J].Popular Science & Technology,2021,23(11):87-90.
点击复制

关于红霉素通过调控RBBP7减轻COPD小鼠 糖皮质激素抵抗的研究()
分享到：

《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
23
期数:
2021年11
页码:
87-90
栏目:
医药与卫生
出版日期:
2021-11-20

文章信息/Info

Title:
Study on Erythromycin Reducing Glucocorticoid Resistance in COPD Mice by Regulating RBBP7
作者:
马　南　何志义　
（广西医科大学第一附属医院，广西 南宁 530021）
关键词:
红霉素; 慢性阻塞性肺疾病; 糖皮质激素抵抗; RBBP7
Keywords:
erythromycin chronic obstructive pulmonary disease glucocorticoid resistance RBBP7
文献标志码:
A
摘要:
目的：验证红霉素通过抑制RBBP7活性改善COPD小鼠糖皮质激素抵抗。方法：使用候选基因法查找与HDAC2、GR相关蛋白RBBP7，使用烟草烟雾暴露建立COPD小鼠模型，HE染色观察红霉素对COPD小鼠肺气肿的影响，rt-PCR验证红霉素对COPD小鼠肺组织中RBBP7、HDAC2、GR基因表达的影响， Westernblot研究红霉素对COPD小鼠肺组织中RBBP7、HDAC2、GR蛋白表达的影响。结果：蛋白质互作网络显示视网膜母细胞瘤结合蛋白7（retinoblastoma binding protein 7，RBBP7）与HDAC2、GR存在互作关系。红霉素可以改善烟草烟雾引起的肺气肿。COPD小鼠肺组织中RBBP7基因表达升高，HDAC2、GR基因表达减少。红霉素可以减少COPD小鼠肺组织中RBBP7基因表达，增加HDAC2、GR基因表达。同时，COPD小鼠肺组织中RBBP7蛋白表达增加，HDAC2、GR、P-GR蛋白表达减少，GR磷酸化水平降低，红霉素可以逆转上述趋势。结论：红霉素可以通过减少COPD小鼠肺组织中RBBP7活性，从而增加HDAC2、GR活性，最终改善COPD小鼠糖皮质激素抵抗。
Abstract:
Objective: To verify that erythromycin improves glucocorticoid resistance in COPD mice by inhibiting RBBP7 activity. Methods: the candidate gene method was used to find the protein RBBP7 related to HDAC2 and GR, and the COPD mouse model was established by tobacco smoke exposure. HE staining was used to observe the effect of erythromycin on emphysema in COPD mice. RT-PCR was used to verify the effect of erythromycin on the gene expression of rbbp7, HDAC2 and GR in the lung tissue of COPD mice. Western blot was used to study the effect of erythromycin on RBBP7, HDAC2 and GR in the lung tissue of COPD mice Effect of GR protein expression. Results: The protein interaction network showed that retinoblastoma binding protein 7 (RBBP7) interacted with HDAC2 and GR. Erythromycin can improve emphysema caused by tobacco smoke. The expression of RBBP7 gene increased and the expression of HDAC2 and GR gene decreased in the lung tissue of COPD mice. Erythromycin can reduce the expression of RBBP7 gene and increase the expression of HDAC2 and GR gene in the lung tissue of COPD mice. At the same time, the expression of RBBP7 protein increased, the expression of HDAC2, GR and P-GR Protein decreased, and the level of GR phosphorylation decreased in the lung tissue of COPD mice. Erythromycin can reverse the above trend. Conclusion: Erythromycin can increase the activities of HDAC2 and GR by reducing the activity of RBBP7 in the lung tissue of COPD mice, and finally improve the glucocorticoid resistance of COPD mice.

相似文献/References:

[1]李瑞祥 陈斯宁.中医药对 COPD 大鼠模型作用机理的研究进展[J].大众科技,2014,16(05):95.
[2]甘间芳.慢性阻塞性肺疾病合并呼吸衰竭应用无创呼吸机的舒适护理效果观察研究[J].大众科技,2014,16(07):128.
　Chronic obstructive pulmonary disease with respiratory failure noninvasiveventilator application effect of comfortable nursing observation research[J].Popular Science & Technology,2014,16(11):128.
[3]杨 赟 梁爱武.中医药对慢性阻塞性肺疾病MMP-9、TIMP-1 影响的研究进展[J].大众科技,2015,15(02):78.
　慢性阻塞性肺疾病；基质金属蛋白酶-9；金属蛋白酶组织抑制-1；气道重塑；中医药[J].Popular Science & Technology,2015,15(11):78.
[4]张 北,梁爱武.中医治疗慢性阻塞性肺疾病稳定期的研究进展[J].大众科技,2019,21(10):53.
　Research Rrogress in the Treatment of Chronic Obstructive Pulmonary Disease by Traditional Chinese Medicine[J].Popular Science & Technology,2019,21(11):53.
[5]贵 磊 陈 平 覃健杰.大环内酯类抗生素对慢性阻塞性肺疾病研究进展[J].大众科技,2018,20(12):50.
　Research Progress on Macrolides Antibiotic in the Therapy of Chronic Obstructive Pulmonary Disease[J].Popular Science & Technology,2018,20(11):50.
[6]孙桂丽,周继红.中医辨证论治慢性阻塞性肺疾病急性加重期的研究进展[J].大众科技,2020,22(04):76.
　Research Progress in the Treatment of Acute Exacerbation of ChronicObstructive Pulmonary Disease by Traditional Chinese Medicine Dialectics[J].Popular Science & Technology,2020,22(11):76.
[7]胡晓玲 陈斯宁 陆 珏.中性粒细胞弹性蛋白酶与慢性阻塞性肺疾病关系的研究进展[J].大众科技,2023,25(1):123.
　Research Progress on the Relationship between Neutrophil Elastase and Chronic Obstructive Pulmonary Disease[J].Popular Science & Technology,2023,25(11):123.

备注/Memo

备注/Memo:
【收稿日期】2021-08-26 【基金项目】广西研究生教育创新计划课题资助项目（YCBZ2019039）。 【作者简介】马南（1987－），男（壮族），广西崇左人，广西医科大学第一附属医院博士研究生，研究方向为呼吸内科。 【通信作者】何志义，广西医科大学第一附属医院主任医师，病区主任，研究方向为呼吸内科。
更新日期/Last Update: 2022-02-14