[1]吕嘉楠 李军生 黄国霞 阎柳娟 马 纪.光谱法对菲与游离DNA的结合模式研究[J].大众科技,2021,23(7):21-24.
 Study on the Binding Mode of Phenanthrene with Free DNA by Spectroscopic Method[J].Popular Science & Technology,2021,23(7):21-24.
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光谱法对菲与游离DNA的结合模式研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
23
期数:
2021年7
页码:
21-24
栏目:
出版日期:
2021-07-20

文章信息/Info

Title:
Study on the Binding Mode of Phenanthrene with Free DNA by Spectroscopic Method
作者:
吕嘉楠12 李军生12 黄国霞12 阎柳娟12 马 纪12
(1.广西科技大学,广西 柳州 545006;2.广西糖资源绿色加工重点实验室,广西 柳州 545006)
关键词:
DNA结合模式光谱法
Keywords:
phenanthrene DNA binding mode spectroscopic method
文献标志码:
A
摘要:
在25℃、pH=7.40的条件下,采用紫外可见光谱法和荧光光谱法对菲(PHE)与游离DNA的结合模式进行了研究。紫外可见光谱分析结果表明PHE与DNA发生了相互作用形成新的复合物。PHE的最大紫外吸收峰出现减色效应并伴随有微弱的红移,这种现象与体系中发生嵌插作用密切相关。荧光光谱分析结果表明,DNA对PHE具有静态猝灭作用,表明PHE与DNA之间形成了稳定的络合物。热力学数据表明在该复合物形成的过程中范德华力和氢键起着主要作用,且该反应在体外是自发进行的。PHE与游离DNA在体外以嵌插方式相互作用,形成了复合物。研究结果可为建立新的基于DNA嵌插的PHE去除法奠定基础。
Abstract:
The binding mode of phenanthrene (PHE) with free DNA was studied in vitro by UV-visible spectroscopy and fluorescence spectroscopy at 25℃ and pH 7.40. The results of UV-visible spectra analysis showed that PHE interacted with DNA to form a new complex. The maximum UV absorption peak of PHE has a subtractive effect with a weak red shift, which is closely related to the intercalation in the system. The results of fluorescence spectra analysis showed that DNA had a static quenching effect on PHE, indicating that a stable complex was formed between PHE and DNA. Thermodynamic data showed that van der Waals forces and hydrogen bonds play an important role in the formation of the complex, and the reaction is spontaneous in vitro. PHE interacts with free DNA by intercalation in vitro to form a complex. The results can lay a foundation for the establishment of a new PHE removal method based on DNA intercalation.

参考文献/References:

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备注/Memo

备注/Memo:
【收稿日期】2021-05-08 【基金项目】国家自然科学基金项目(21966008);广西自然科学基金项目(2018GXNSFDA281030,2020GXNSFAA159021)。 【作者简介】吕嘉楠(1996-),广西科技大学生物与化学工程学院研究生,研究方向为生物分子的化学修饰与功能研究。 【通信作者】李军生,广西科技大学生物与化学工程学院教授,硕士研究生导师,研究方向为生物分子的化学修饰与功能研究。
更新日期/Last Update: 2021-10-22