[1]麦庭瑜 吴春燕 黄素素 李晟乐 韦元锋 谭 超 何永华.低浓度双酚 A 对小鼠睾丸支持细胞的损伤作用及机制研究[J].大众科技,2020,22(07):72-75.
 Study on the Damage Effect and Mechanism of Low Concentration Bisphenol A on Testicular Sertoli Cells of Mouse[J].Popular Science & Technology,2020,22(07):72-75.
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低浓度双酚 A 对小鼠睾丸支持细胞的损伤作用 及机制研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
22
期数:
2020年07
页码:
72-75
栏目:
医药与卫生
出版日期:
2020-07-20

文章信息/Info

Title:
Study on the Damage Effect and Mechanism of Low Concentration Bisphenol A on Testicular Sertoli Cells of Mouse
作者:
麦庭瑜 吴春燕 黄素素 李晟乐 韦元锋 谭 超 何永华
(桂林医学院公共卫生学院,广西 桂林 541001)
关键词:
睾丸支持细胞双酚 A痛敏肽CFTRp-CREB
Keywords:
testicular Sertoli cells bisphenol A nociceptin CFTR p-CREB
文献标志码:
A
摘要:
目的:观察低浓度双酚 A(BPA)对小鼠睾丸支持细胞存活率的影响,并探讨其机制。方法:使用 DMEM/F12 培养液培养小鼠睾丸支持细胞 TM4 细胞系传代增长至对数期时经不同剂量 BPA 染毒 2 h、8 h,MTT 法检测细胞存活率、微量 酶标法检测 LDH 活性、ELISA 法检测囊性纤维化跨膜转导调节因子(CFTR)与痛敏肽的表达水平、免疫印迹法检测 CREB 表 达水平。采用方差分析比较不同 BPA 浓度、染毒时间的效应。结果:与对照组相比,BPA 染毒组睾丸支持细胞存活率均显著 降低(χ2=28.6,P<0.01),1000 μg/mL、24 h 染毒组的细胞存活率下降约 85%;LDH 活性在 5 μg/mL、2 h 染毒组中下降约 69%, 与 5 μg/mL、2h 染毒组相比 10 μg/mL、2h 染毒组中 LDH 活性上升约 175%;而 CFTR 表达水平、痛敏肽表达水平、CREB 表达 水平均无明显差异。结论:低浓度 BPA 降低小鼠睾丸支持细胞存活率,可能通过下调支持细胞内 CREB 表达,干扰 P-CREB 对 细胞的调控作用,降低对精子细胞的支持作用。
Abstract:
Objective: To observe the effects of low concentration of bisphenol A (BPA) on the survival of Sertoli cells in mice and explore its mechanism. Methods: Using DMEM/F12 cultured mouse testicular support cell TM4 cell line transmission to the interstitial period by different doses of BPA dyes 2 h, 8 h, MTT method was used to detect cell survivor rate, LDH activity was measured by microenzyme method, the expression levels of regulatory factor of cystic fibrosis transmenbrane transdrction (CFTR) and pain sensitive peptide were detected by ELISA, the expression level of CREB was detected by western blot. The effects of different BPA concentrations and drug-dying time were compared by using anova. Results: Compared with the control group, the survival rate of Sertoli cells in BPA exposed group was decreased significantly (χ2=28.6, P<0.01), the survival rate of Sertoli cells in 1000 μg/mL and 24 hours cell survival rate of the exposed group decreased by about 85% the expression level of CFTR protein in Sertoli cells was not statistically significant compared with the control group, the LDH activity in 5 μg/mL and 2 h exposed group decreased by about 69%, and the LDH activity in 10 μg/mL and 2 h exposed group increased by 175% compared with 5 μg/mL/2 h exposed group the expression level of pain-sensitive peptide in Sertoli cells was not statistically significant the expression level of CREB protein in Sertoli cells was not statistically significant. Conclusion: Low concentration of BPA can reduce the survival rate of Sertoli cells in mice, which may interfere with the regulation of p-CREB on cells and decrease the supporting effect of sperm cells by down regulating the expression of CREB in Sertoli cells.

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备注/Memo

备注/Memo:
【收稿日期】2020-05-02 【作者简介】麦庭瑜(1995-),男,广西贺州人,桂林医学院在读硕士研究生,研究方向为环境卫生。
更新日期/Last Update: 2020-11-16