[1]蒋 鹏 高宏君 吴玲玲 武 桢.复方扶芳藤合剂对糖尿病模型大鼠 BMSCs 动员的影响[J].大众科技,2020,22(07):44-47.
　Effect of Fufangteng Combination Formula on BMSCs Mobilization in Diabetic Rats[J].Popular Science & Technology,2020,22(07):44-47.
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复方扶芳藤合剂对糖尿病模型 大鼠 BMSCs 动员的影响()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
22
期数:
2020年07
页码:
44-47
栏目:
医药与卫生
出版日期:
2020-07-20

文章信息/Info

Title:
Effect of Fufangteng Combination Formula on BMSCs Mobilization in Diabetic Rats
作者:
蒋 鹏 高宏君 吴玲玲 武 桢
（广西中医药大学附属瑞康医院，广西 南宁 530011）
关键词:
复方扶芳藤合剂; 骨髓间充质干细胞; 粒细胞集落刺激因子; 中医药
Keywords:
Fufangteng combination formula bone marrow mesenchyml stem cell granulocyte colony stimulating factor traditional Chinese medicine
文献标志码:
A
摘要:
目的：探究复方扶芳藤合剂对糖尿病模型大鼠骨髓来源干细胞 BMSCs 动员的影响。方法：采用糖尿病模型大 鼠作为实验模型，以随机数表法将糖尿病模型大鼠随机分为模型组、复方扶芳藤合剂组和西药组。模型组常规饲养；复方扶芳 藤合剂组予复方扶芳藤合剂 3.1 mL/d 灌胃，一天灌胃两次，持续干预 14 天。西药组予集落刺激因子皮下注射，每两天注射一 次，每次 10 μg/(kg×d)，共计 8 次 14 天。以 SD 大鼠作为空白组。于干预 14 d 进行指标观察，流式细胞仪检测外周血中骨髓间 充质干细胞（BMSCs）数量；ELISA 检测外周血粒细胞集落刺激因子（G-CSF）含量变化。结果：流式细胞仪分析显示，与模 型组相比较，复方扶芳藤合剂组外周血 BMSCs 数量明显加（P＜0.05）。ELISA 检测显示，与模型组相比较，复方扶芳藤合剂治 疗 14 d 后，外周血 G-CSF 明显增加（P＜0.05）。结论：复方扶芳藤合剂能发挥糖尿病模型大鼠骨髓间充质干细胞动员的作用， 其机制可能与增加糖尿病大鼠外周血 G-CSF 含量有关。
Abstract:
Objective: To explore the effect of Fufangteng combination formula on BMSCs mobilization of bone marrow-derived stem cells in diabetic rats. Methods: Using the diabetes model rat as the experimental model, the diabetic rats were randomly divided into model group, compound Fufangteng combination formula group and western medicine group. The model group was given routine feeding the Fufangteng combination formula group was gavage to the Fufangteng combination formula 3.1 mL/d, twice a day, and the intervention continued for 14 days. The western medicine group was injected with colony stimulating factor subcutaneously once every two days, 10 μg/ (kgd) for 8 times for 14 days. SD rats were used as blank group. After 14 days of intervention, the number of bone marrow mesenchymal stem cells (BMSCs) in peripheral blood was detected by flow cytometry, changes of granulocyte colony stimulating factor (G-CSF) in peripheral blood were detected by ELISA. Results: The flow cytometer analysis showed that compared with the model group, the number of BMSCs in peripheral blood of the Fufangteng combination formula group was significantly increased (P＜0.05). ELISA tests showed that compared with the model group, G-CSF in peripheral blood increased significantly after 14 days of treatment with Fufangteng combination formula (P＜0.05). Conclusion: Fufangteng combination formula has a certain mobilization effect on BMSCs in diabetic rats, and its mechanism may be related to the increase of G-CSF content in peripheral blood of diabetic rats.

参考文献/References:

[1] International Diabetes Federation. IDF Diabetes Atlas (9th Edition)[M]. Brussels: International Diabetes Federation, 2019. [2] Read S H, Kerssens J J, McAllister DA, et al. Trends in type 2 diabetes incidence and mortality in Scotland between 2004 and 2013[J]. Diabetologia. 2016, 59: 2106-2113. [3] Wang, Z X, Cao J X, Li D, et al. Clinical efficacy of autologous stem cell transplantation for the treatment of patients with type 2 diabetes mellitus: a meta-analysis[J]. Cytotherapy, 2015, 17(7): 956-968. [4] Shree N, Bhonde R.R. Metformin preconditioned adipose derived mesenchymal stem cells is a better option for the reversal of diabetes upon transplantation[J]. Biomed Pharmacother, 2016, 84: 1662-1667. [5] Ianus A, Holz G G, Theise N D, et al. In vivo derivation of glucose-competent pancreatic endocrine cells from bone marrow without evidence of cell fusion[J]. The Journal of Clinical Investigation, 2003, 111(6): 843-850. [6] 孙吉平，尹爱萍，吕晶，等. 骨髓间充质干细胞移植对 糖尿病大鼠的治疗作用[J]. 中华器官移植杂志，2010， 31(1): 46-49. [7] Xie Q P, Huang H, Xu B, et al. Human bone marrow mesenchymal stem cells differentiate into insulinproducing cells upon microenvironmental manipulation in vitro[J]. Differentiation: Research in Biological Diversity, 2009, 77(5): 483-491. [8] 张雨，常军英，张宁，等. 复方扶芳藤合剂对小鼠外周血 干细胞动员作用的研究[J]. 中国实验方剂学杂志，2012， 18(7): 212-215. [9] 华臻，杨俊锋，潘娅岚，等. 补肾中药促进骨髓间充质 干细胞成骨分化的研究进展[J]. 中华中医药杂志，2015， 30(9): 3222-3226. [10] 高璐，郑洪新，陈谊敬，等. 补肾中药成分配伍调控 RUNX2、OSX 对大鼠 BMSCs 成骨分化的影响[J]. 世界 中西医结合杂志，2014，9(4): 425-429. [11] 赖满香，冯娟，陈侠，等. 巴戟天多糖对大鼠骨髓间充质 干细胞增殖和骨向分化的影响[J]. 中国医药科学，2018， 8(7): 33-36. [12] 卫荣，温欣，王莉，等. 中药补肾活血方提取物促进 SD 大鼠骨髓间充质干细胞增殖作用的最佳浓度[J]. 中国组 织工程研究，2019，23(5): 691-696. [13] 黄宏妙，唐红珍，李灵，等. 复方扶芳藤合剂对 D-半乳 糖所致衰老小鼠的抗衰老作用[J]. 中成药，2016，38(8): 1814-1817. [14] 周倍伊，吴亚姗，周围，等. 大鼠骨髓间充质干细胞培 养鉴定及复方扶芳藤合剂含药血清对细胞增殖的影响 [J]. 广西中医药，2020(2): 56-61. [15] 周倍伊，万娟，周围，等. 复方扶芳藤合剂含药血清对 大鼠体外模拟“造血龛”中造血干细胞增殖的影响[J].广 西中医药，2020，43(1): 63-68. [16] Han W H, Xing J C, Ren H M, et al. Lung fibrosis C-score and expression of surfactant protein-D in early stage of coal worker pneumoconiosis[J]. Journal of Public Health and Preventive Medicine, 2009, 20(1): 4-7. [17] Petit I, Szyper-Kravitz M, Nagler A, et al. G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4[J]. Nature Immunology, 2002, 3(7): 687-694. [18] 谢晓强，吕碧锋，章振保，等. G-CSF 动员骨髓干细胞向 缺血-再灌注肾脏归巢并促进肾脏修复的研究[J]. 中华急 诊医学杂志，2012，21(5): 491-496. [19] Harada M, Qin Y, Takano H, et al. G-CSF prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes[J]. Nature Medicine, 2005, 11(3): 305-311.

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备注/Memo

备注/Memo:
【收稿日期】2020-05-05 【基金项目】广西自然科学基金面上项目（2017GXNSFAA198080）；广西中医药大学 2018 年广西一流学科建设项目重 点课题（2018XK085）；广西壮族自治区中青年教师基础能力提升项目（2019KY0317）；广西中医药大学 2019-2011 年广西 一流学科建设项目青年基金项目（2019XK176）。 【作者简介】蒋鹏，广西中医药大学附属瑞康医院住院医师，硕士，研究方向为器官移植泌尿外科。
更新日期/Last Update: 2020-11-16