[1]何龙凉 陈延伟 李小琴 杨怀军 韦新红.GC-MS 法同时测定抗草甘膦转基因大豆中多种除草剂的残留量[J].大众科技,2019,21(12):17-19.
　Simultaneous Determination of Herbicide Residues in Glyphosate ResistantTransgenic Soybeans by GC-MS[J].Popular Science & Technology,2019,21(12):17-19.
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GC-MS 法同时测定抗草甘膦转基因大豆中多种除草剂的残留量()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
21
期数:
2019年12
页码:
17-19
栏目:
轻工与化工
出版日期:
2019-12-20

文章信息/Info

Title:
Simultaneous Determination of Herbicide Residues in Glyphosate ResistantTransgenic Soybeans by GC-MS
作者:
何龙凉 陈延伟 李小琴 杨怀军 韦新红
（中华人民共和国防城海关，广西 防城港 538001）
关键词:
抗草甘膦转基因大豆; 气相色谱-质谱联用; 除草剂
Keywords:
glyphosate resistant transgenic soybean;  gas chromatography-mass spectrometry;  herbicide
文献标志码:
A
摘要:
利用气相色谱-质谱联用法(Gas Chromatography - tandem Mass Spectrometry，GC-MS)同时测定防城港口岸进境的不同原产国抗草甘膦转基因大豆（Roundup Ready Soybean，简称 RR 大豆）中氟乐灵、甲草胺、异丙甲草胺、吡氟禾草灵、烯禾啶的残留量。方法为采集原产自美国、巴西、阿根廷和乌拉圭的 RR 大豆各 3 批，样品磨碎过筛以乙腈（含 1%乙酸）为萃取剂用加速萃取仪进行萃取，萃取液过 Envi-18 固相萃取柱，再经 Sep-Pak Carbon/ NH2 双层柱进一步净化后，加入 20 μL 环氧七氯内标溶液，用正己烷定容至 1 mL 供 GC-MS 分析。结果表明，氟乐灵、甲草胺、异丙甲草胺、吡氟禾草灵和烯禾啶在不同浓度范围内的线性良好（r＞0.99），样品加标平均回收率为77.4%～92.5%，相对标准偏差为4.5%～10.1%，定量下限0.01～0.05 mg/kg。该分析方法，方法准确可靠，回收率和精密度符合残留检测要求，对提高进口转基因大豆监管工作效率和进一步分析评估转基因大豆的食用安全性有一定的参考价值。
Abstract:
Gas Chromatography-Tandem Mass Spectrometry (GC-MS) was used to simultaneously determine the residues oftrifluralin, metolachlor, metolachlor, picroflofenac, and echloridine in Roundup Ready Soybean (RR soybean) from different countries oforigin that had entered Fangchenggang port. Methods: Three batches of RR soybeans from the United States, Brazil, Argentina andUruguay were collected. The samples were ground and sieved with acetonitrile (containing 1% acetic acid) as extractant and extractedwith an accelerated extractor. The extract was passed through the Envi-18 solid phase extraction column. The column was furtherpurified by Sep-Pak Carbon/NH2 double-layer column, 20 μL of epoxy heptachlor standard solution was added, and the volume wasadjusted to 1 mL with n-hexane for GC-MS analysis. Results: The linearity of trifluralin, alachlor, metolachlor, pyridoxine andmethotrexate in different concentration ranges was good (r＞0.99). The average recovery of standard addition was 77.4%~92.5%. Therelative standard deviation is 4.5% to 10.1%, and the lower limit of quantification is 0.01~0.05 mg/kg. The analysis method is accurateand reliable, the recovery rate and precision meet the requirements of residue detection. It has certain reference value for improving thesupervision efficiency of imported genetically modified soybeans and further analyzing and evaluating the edible safety of geneticallymodified soybeans.

参考文献/References:

[1] 崔宁波，张正岩.转基因大豆研究及应用进展[J]. 西北农业学报，2016，25(8): 1111-1124.[2] 苏明明，张旭东，那晗，等.凝胶渗透色谱-气相色谱-质谱联用快速测定大豆中克百威、乙草胺、甲草胺、异丙甲草胺、氟乐灵的残留量[J]. 质谱学报，2012，33(1): 37-41.[3] GB/T 5009.172-2003，大豆、花生、豆油、花生油中的氟乐灵残留量的测定[S]. 北京: 中华人民共和国卫生部，2003.[4] GB/T 5009.174-2003，花生、大豆中异丙甲草胺残留量的测定[S]. 北京: 中华人民共和国卫生部，2003.[5] GB/T 5009.142-2003，植物性产品中吡氟禾草灵、精吡氟禾草灵残留量的测定[S]. 北京: 中华人民共和国卫生部，2003.

备注/Memo

备注/Memo:
【收稿日期】2019-10-15【作者简介】何龙凉（1985－），男，中华人民共和国防城海关农艺师，研究方向为食品及农产品检测。
更新日期/Last Update: 2020-03-10