[1]韦月早,杨世联,黄艳芳,等.高效液相色谱法测定多种维生素多种矿物质片中的维生素 D 3 含量[J].大众科技,2019,21(06):40-42.
　Determination of Vitamin D 3 in Multiple Vitamins and Minerals Tabletby High Performance Liquid Chromatography[J].Popular Science & Technology,2019,21(06):40-42.
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高效液相色谱法测定多种维生素多种矿物质片中的维生素 D 3 含量()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
21
期数:
2019年06
页码:
40-42
栏目:
轻工与化工
出版日期:
2019-06-20

文章信息/Info

Title:
Determination of Vitamin D 3 in Multiple Vitamins and Minerals Tabletby High Performance Liquid Chromatography
作者:
韦月早1;  杨世联 2;  黄艳芳 2;  韦艳妮 2
（1.广西壮族自治区产品质量检验研究院，广西 南宁 530007；2.南宁富莱欣生物科技有限公司，广西 南宁 530007）
关键词:
高效液相色谱法; 多种维生素多种矿物质片; 维生素 D 3
Keywords:
high performance liquid chromatography;  multiple vitamins and minerals tablet;  vitamin D 3
文献标志码:
A
摘要:
建立高效液相色谱法快速测定多种维生素多种矿物质片中维生素 D 3 的含量，并采用加标回收率试验进行方法确认。样品在 0.2%的氨水中用碱性蛋白酶破坏维生素 D 3 的包埋物质，用乙醇浸提，正己烷萃取游离出维生素 D 3 。供试品溶液用正相液相色谱法净化，反相液相色谱法分离。维生素 D 3 在 0.0498μg/mL～0.9963μg/mL 浓度范围内线性关系良好（r＝0.9999），平均加标回收率为 97.78％，RSD 为 3.26％，精密度和稳定性良好，RSD 分别为 2.69％和 1.58％。该方法准确、快速，可作为多种维生素多种矿物质片中维生素 D 3 含量的测定方法。
Abstract:
A rapid method for the determination of vitamin D 3 in multiple vitamins and minerals tablet by high performance liquidchromatography was established. The method was confirmed by standard-added recovery test. In 0.2% ammonia water, the encapsulatedsubstance of vitamin D 3 was destroyed by alkaline protease. Vitamin D 3 was extracted by ethanol and n-hexane. The sample solution waspurified by normal phase liquid chromatography and separated by reversed phase liquid chromatography. The linear relationship of vitaminD 3 was good in the concentration range of 0.0498μg/mL to 0.9963μg/mL (r＝0.9999). The average recovery of standard addition was97.78%, RSD was 3.26%. The precision and stability of vitamin D 3 were good, RSD were 2.69% and 1.58%, respectively. The method isaccurate and rapid, and can be used for the determination of vitamin D3 in multiple vitamins and minerals tablet.

参考文献/References:

[1] 周建烈.维生素D 健康密码[M].北京:中国工业出版社,2013.[2] 陈君石.维生素矿物质补充剂在疾病防治中的临床应用:专家共识[M].北京:人民卫生出版社,2009.[3] 国家药典委员会.中国药典(四部)[M].北京:中国医药科技出版社,2015.[4] GB 5009.82-2016,食品安全国家标准.食品中维生素 A、D、E 的测定[S].北京:中国标准出版社,2016.[5] GB 5413.9-2010,食品安全国家标准 婴幼儿配方食品和乳粉维生素A、D、E 的测定[S].北京:中国标准出版社,2010.[6] 张明晶,关士海.保健食品中维生素 D 3 测定方法的改进[J].中国医药指南,2012,10(31):102-103.[7] 马莉,王英新,王龙江,等.方碳酸钙泡腾颗粒中维生素 D 3含量测定的方法改进[J].药学研究,2017,7(36):393-395.[8] 周华.高效液相色谱法测定阿仑膦酸钠维生素 D 3 片中维生素D 3 的含量及有关物质[J].亚太传统医药,2012,8(6):51-52.[9] 徐硕,吴学军,徐文峰,等.高效液相色谱法快速测定维生素D 3 软胶囊中维生素D 3 的含量[C].中国药学会第三届药物检测质量管理学术研讨会,2016.

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备注/Memo

备注/Memo:
【收稿日期】2019-04-09【作者简介】韦月早（1984－），供职于广西壮族自治区产品质量检验研究院，从事环境检测工作。
更新日期/Last Update: 2020-01-21