[1]谭伟东 朱艳霞 柯 芳 叶志文 钟一雄 董青松.毛鸡骨草种子四唑染色法的研究[J].大众科技,2019,21(03):25-26.
　Study on Triphenyl Tetrazolium Chloride Test of Abrus mollis Handce Seeds[J].Popular Science & Technology,2019,21(03):25-26.
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毛鸡骨草种子四唑染色法的研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
21
期数:
2019年03
页码:
25-26
栏目:
出版日期:
2019-03-20

文章信息/Info

Title:
Study on Triphenyl Tetrazolium Chloride Test of Abrus mollis Handce Seeds
作者:
谭伟东1 朱艳霞 2 柯 芳 2 叶志文 1 钟一雄 1 董青松 2
（1.广西玉林制药集团有限责任公司，广西 玉林 537001；2.广西药用植物园，广西 南宁 530023）
关键词:
毛鸡骨草; 种子; 四唑染色法; 发芽
Keywords:
Abrus mollis Hance;  seeds;  triphenyl tetrazolium chloride test;  germination
文献标志码:
A
摘要:
为完善毛鸡骨草种子质量检验方法，对其种子的生活力测定方法——四唑染色法进行了研究。通过考察种子预湿时间及方法、种子染色前处理、染色液浓度、染色温度、染色时间，确定了毛鸡骨草种子四唑染色条件。毛鸡骨草种子四唑染色方法为净种子经破除硬实后，于 30℃恒温水浸种 5h～6h 预湿，剥皮，1.0%TTC 溶液 30℃黑暗染色 5h～6h。结果表明，该方法对不同毛鸡骨草种子的染色率与发芽试验结果基本一致。毛鸡骨草种子的四唑测定稳定可靠，可代替发芽试验。
Abstract:
To improve the methods of seeds quality test of Abrus mollis Hance, the technique of triphenyl tetrazolium chloride (TTC)test was studied. The conditions of TTC test for Abrus mollis seeds were determined by investigating the way of wetting and pretreatment,the concentration of TTC solution, the dyeing temperature and time. The TTC test method of Abrus mollis Hance seeds are following: Afterthe hard seeds were broken, the seeds were soaked in constant temperature water at 30°C for 5h-6h, and then peeled. The seeds were dyed in1.0% TTC solution at 30°C for 5h-6h in darkness. The results showed that the dyeing rate of different Abrus mollis seeds was basically thesame as that of germination test. The TTC test of Abrus mollis Hance seeds is reliable, which can replace the germination test.

参考文献/References:

[1] 广西壮族自治区卫生厅.广西中药材标准[M].南宁:广西科学技术出版社,1990.[2] GB/T 3543.7-1995.中华人民共和国国家标准.农作物种子检验规程 其他项目[S].北京:中国标准出版社,1995.[3] 胡晋.种子生活力测定原理和方法[M].北京:中国农业出版社,2009.[4] 支巨振.GB/T3543.1~3543.7-1995 农作物种子检验规程实施指南[M].北京:中国标准出版社,2004.[5] 董青松,陈章源,何俊兵,等.毛相思子种子发芽试验研究[J].种子,2010,29(4):12-15.

备注/Memo

备注/Memo:
【收稿日期】2019-01-16【基金项目】广西科技成果转化与推广计划项目（桂科转 1599004-3）。【作者简介】谭伟东（1989－），男，广西玉林制药集团有限责任公司助理工程师，从事药用植物栽培及种子标准研究。【通信作者】董青松，供职于广西药用植物园。
更新日期/Last Update: 2020-01-16