[1]何婕培.条斑紫菜对潮霉素敏感性研究[J].大众科技,2018,20(11):19-21.
　Studies on the Sensitivity of Pyropia Yezoensis to Hygromycin[J].Popular Science & Technology,2018,20(11):19-21.
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条斑紫菜对潮霉素敏感性研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
20
期数:
2018年11期
页码:
19-21
栏目:
轻工与化工
出版日期:
2018-11-20

文章信息/Info

Title:
Studies on the Sensitivity of Pyropia Yezoensis to Hygromycin
作者:
何婕培
（中国海洋大学海洋生命学院，山东 青岛 266003）
关键词:
条斑紫菜; 敏感性; 潮霉素
Keywords:
Pyropia yezoensis;  sensitivity;  Hygromycin
文献标志码:
A
摘要:
条斑紫菜作为重要的经济海洋藻类，已在基因工程领域中做了诸多研究，包括核遗传转化和质体遗传转化，并且已经在紫菜中实现稳定表达和瞬时表达。文章研究了条斑紫菜对潮霉素的敏感性，获得筛选条件。结果表明，潮霉素对条斑紫菜叶状体的筛选浓度为2mg/mL，其中RZ 品系筛选时间为15 天；野生型品系为14 天。对条斑紫菜原生质体的筛选浓度为0.5mg/mL，其中RZ 品系筛选时间为7 天；野生型品系为6 天。该结果有助于基因编辑系统CRISPR/Cas9 技术在条斑紫菜研究中的应用。
Abstract:
Pyropia yezoensis is one of the most economically important marine species of the world. Many researches about it have been done in genetic engineering including establishing the platforms of nuclear transformation platform and plastid transformation platform and realize stable and transient expression in Pyropia yezoensis. The sensitivity of the gametophyte cells of Pyropia yezoensis to Hygromycin is 2.0 mg/mL, all cells of RZ died after 15 days while all cells of wild species died after 14 days; and that of protoplasts is 0.5 mg/mL, all cells of RZ died after 7 days while all cells of wild species died after 6 days. It is helpful for the application of CRISPR/Cas9 geno-editing systems in Pyropia yezoensis.

参考文献/References:

【参考文献】 [1] 宫倩红.条斑紫菜原生质体转基因的研究[D].青岛:中国海洋大学,2006. [2] 刘红全.条斑紫菜转基因研究[D].青岛:中国海洋大学,2003. [3] Uji T,Hirata R,Fukuda S,et al.A codon-optimized bacterial antibiotic gene used as selection marker for stable nuclear transformation in the marine red alga Pyropia yezoensis [J].Marine biotechnology,2014,16(3):251-255. [4] Takahashi M,Mikami K,Mizuta H,et al.Identification and efficient utilization of antibiotics for the development of a stable transformation system in Porphyra yezoensis (Bangiales,Rhodophyta)[J].Journal of Aquaculture Research & Development,2011,6(1):519-523. [5] Kong F,Zhao H,Liu W,et al.Construction of plastid expression vector and development of genetic transformation system for the seaweed Pyropia yezoensis[J].Marine Biotechnology, 2017,19(2):147-156. [6] 王素娟,张小平,徐志东,等.坛紫菜营养细胞和原生质体培养的研究[J].海洋与湖沼,1986,17(3):217-221. [7] 沈怀舜,王素娟.紫菜体细胞对抗生素的敏感性实验[J].海洋科学,1998,22(1):67-69. [8] 王萍,成锋,滕亚娟,等.抗生素抑制农杆菌的效果及对条斑紫菜生长发育的影响[J].水产科学,2009,28(7):405-408. [9] 赵扬,李秀,左正宏,等.坛紫菜叶状体体细胞对抗生素敏感性的研究[J].台湾海峡,2004,23(4):496-499. [10] Jiang W,Brueggeman A J,Horken K M,et al.Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii[J].Eukaryotic cell,2014,13(11): 1465-1469. [11] Nymark M,Sharma A K,Sparstad T,et al.A CRISPR/Cas9 system adapted for gene editing in marine algae[J]. Scientific reports,2016,6:24951.

备注/Memo

备注/Memo:
【收稿日期】2018-09-12 【作者简介】何婕培（1992－），女，广西南宁人，中国海洋大学海洋生命学院在读硕士研究生，研究方向为藻类遗传学。
更新日期/Last Update: 2018-12-19