[1]张笮晦 李石兰 童永清 钱信怡.肉桂叶生物转化的放大实验及产物的 抑菌活性研究[J].大众科技,2018,20(08):83-85，89.
　Studies on Magnifying Experiment of Biotransformation of CinnamomumLeaves and Antimicrobial Activity of the Product[J].Popular Science & Technology,2018,20(08):83-85，89.
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肉桂叶生物转化的放大实验及产物的 抑菌活性研究()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
20
期数:
2018年08期
页码:
83-85，89
栏目:
医药与卫生
出版日期:
2018-08-20

文章信息/Info

Title:
Studies on Magnifying Experiment of Biotransformation of CinnamomumLeaves and Antimicrobial Activity of the Product
作者:
张笮晦1; 2 李石兰3 童永清3 钱信怡3
（1.广西中医药大学，广西 南宁 530200； 2.广西中药药效研究重点实验室，广西 南宁 530200； 3.广西庚源香料有限责任公司，广西 东兴 538100）
关键词:
肉桂醇; 生物转化; 放大实验; 抑菌活性
Keywords:
cinnamyl alcohol;  biotransformation;  magnifying experiment;  antibacterial activity
文献标志码:
A
摘要:
文章研究鞘氨醇单胞菌Sphingomonas sp MX18 转化肉桂叶制备天然肉桂醇的3L 摇瓶放大实验及产物肉桂醇的抑菌活性。通过单因素实验对放大实验进行条件优化，采用滤纸片法考察产物肉桂醇的抑菌活性。结果表明：3L 摇瓶放大实验的最优工艺为： 接种量为100 mL/L，摇瓶装液量为660 mL，转化4 d，肉桂醇得率为0.79 %，该转化反应在3L 摇瓶放大实验是可行的。产物肉桂醇对金黄色葡萄球菌、大肠杆菌、绿脓杆菌具有一定的抑菌活性，其中对金黄色葡萄球菌的抑制作用最强。
Abstract:
The magnifying experiment of natural cinnamyl alcohol transformed by Sphingomonas sp MX18 from cinnamon leaves in 3 litre shaking flask was studied, and the antibacterial activity of cinnamyl alcohol was also studied. Single-factor experiments were conducted to optimize the magnifying experiment. Through filter paper method, the antibacterial activity of the conversion product cinnamyl alcohol was examined. The results showed that the optimal process of magnifying experiment in 3 litre shaking flask was as follows: the amount of strain MX18 seed fluid was 100 mL in per litre fermentation medium, the volume of shake flask was 660 mL, transformed for 4 days in 3L shake flask. Under this condition, the yield of cinnamyl alcohol was 0.79%. It seemed that the magnifying experiment in 3 litre shaking flask is feasible. Cinnamyl alcohol has certain antibacterial activities against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Among them, the inhibition for Staphylococcus aureus is the strongest.

参考文献/References:

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备注/Memo

备注/Memo:
【收稿日期】2018-06-05 【基金项目】广西自然科学基金项目（2015GXNSFAA139102）；广西中药药效研究重点实验室（16-380-29）。 【作者简介】张笮晦（1979－），女，广西荔浦人，广西中医药大学药学院讲师，从事天然药物的生物化工、农作物废弃物功能成分的开发与研究工作。
更新日期/Last Update: 2018-09-06