[1]李静 白广红 魏墚铉 曹琳 王卓.微孔板法检测痰标本结核分枝杆菌及耐药多因素分析[J].大众科技,2018,20(06):82-84，109.
　Detection of Mycobacterium tuberculosis in sputum using microplate methodand the multi-factor analysis of its drug resistance[J].Popular Science & Technology,2018,20(06):82-84，109.
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微孔板法检测痰标本结核分枝杆菌及耐药多因素分析()
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
20
期数:
2018年06期
页码:
82-84，109
栏目:
医药与卫生
出版日期:
2018-06-20

文章信息/Info

Title:
Detection of Mycobacterium tuberculosis in sputum using microplate methodand the multi-factor analysis of its drug resistance
作者:
李静 白广红 魏墚铉 曹琳 王卓
关键词:
微孔板法; 结核分枝杆菌; 药敏试验
Keywords:
Microplate method;  Mycobacterium Tuberculosis;  drug susceptibility test
文献标志码:
A
摘要:
目的：评价微孔板法用于检测痰标本结核分枝杆菌（MTB）耐药的临床价值及探讨结核分枝杆菌耐药的影响因素。方法：采用MGIT960 快速药敏法（MGIT 法）和微孔板法对298 例肺结核患者痰标本中的MTB 进行药敏试验，并对其耐药多因素进行Logistic 回归分析。结果：298 例痰液标本中有人结核分枝杆菌293 例，牛结核分枝杆菌5 例，对比差异无统计学意义（P＞0.05）。与MGIT 法测定结果比较，微孔板法检测利福平耐药性的敏感度为95.2%，特异度为97.4%；异烟肼耐药性的敏感度为91.8%，特异度为92.3%，Kappa值分别为0.689 及0.653。多因素Logistic 回归分析结果显示，吸烟史、诊断延误与合并HIV 对MTB 耐药性影响显著（P＜0.05）。结论：微孔板法检测痰标本中MTB 耐药性与MGIT960 快速药敏法有较高一致性。
Abstract:
Objective: To evaluate the clinical application value of microplate method on the detection of Mycobacteriumtuberculosis (MTB) in sputum and to explore the influence factors of drug resistance of MTB. Methods: The drug resistance of MTB in298 sputum samples from tuberculosis patients were tested using MGIT960 rapid susceptibility method (MGIT method)and Microplatemethod respectively and the drug resistance multi-factors were analyzed by Logistic regression. Results: Among the 298 sputum samples,293 samples carry human MTB, the other 5 samples carry bacillus tuberculosis, and the contrast differences were not significant (P＞0.05). Compared with the MGIT method, the sensitivity and specificity of microplate method for detecting rifampicin were 95.2% and97.4% respectively, and that for detecting isoniazid resistance were 91.8% and 92.3% respectively. The Kappa value were 0.689 and0.653, respectively. The results of multi-factor logistic regression analysis indicated that MTB drug resistance rate was significantlyinfluenced by smoking history, diagnosis delay and merger of HIV (P＜0.05). Conclusion: The results of the Microplate Method showedexcellent concordance with those of MGIT method in detecting the resistance rate of Mycobacterium Tuberculosis of sputum samples.

参考文献/References:

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备注/Memo

备注/Memo:
【作者简介】李静，女，陕西省结核病防治院检验师，从事临床检验研究。
更新日期/Last Update: 2018-07-12