[1]许子竞 庞 国.HPLC 法测定双山颗粒中绿原酸的含量[J].大众科技,2014,16(01):35.
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HPLC 法测定双山颗粒中绿原酸的含量
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《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
第16卷
期数:
2014年01期
页码:
35
栏目:
轻工与化工
出版日期:
2014-03-29

文章信息/Info

Title:
Determinations of chlorogenic acid in Shuangshan Granules by HPLC
文章编号:
1008-1151(2014)01-0035-03
作者:
许子竞 庞 国
广西金秀圣堂药业有限责任公司，广西 金秀 545700
关键词:
绿原酸; HPLC; 双山颗粒; 含量测定
Keywords:
Chlorogenic acid;  HPLC;  Shuangshan Granules;  determination
分类号:
R284
文献标志码:
B
摘要:
目的：建立 HPLC 测定双山颗粒中山绿茶中绿原酸含量的方法。方法：ODS-C18 色谱柱（5μm，4.6nm×25cm）； 以乙腈-0.01mol/L 磷酸氢二钾溶液（冰醋酸调节至 pH 值至3.0）（6:94）流动相，流速 1.0mL/min,检测波长 326nm，柱温 30℃。 结果：绿原酸在 0.38～1.428μg范围内，线性关系良好。回归方程为 Y=1.7253×104-7.0558,(r=0.9997)，平均加样回收率为 97.6%， RSD 为 1.25%（n=5)。结论：所建立的绿原酸 HPLC 含量检测方法操作简便、快速，灵敏度高，结果准确可靠，专属性强，重 现性好，可用于双山颗粒制剂的质量控制
Abstract:
Objective: To establish a determination method of Chlorogenic acid in Shuangshan Granules by HPLC. Methods: Symmertry C18 column (5μm，4.6nm×25cm);The mobile phase consisted of Acetonitrile–0.01mol/L dipotassium hydrogen phosphate solution (acetic acid adjusted ph to 3.0) ( 6:94) and the detective wavelength was at 326nm.The column temperature was at 30℃ and the flow rate was 1 mL/min .Results: The methodological study showed that a good linear correlation existed in the range of 0.38～1.428μg of Chlorogenic acid. Equation Y=1.7253×104-7.0558,(r=0.9997).The average recovery was 97.6% and RSD =1.25%（n=5).Conclustion: The method of HPLC is simple, precise and reproduciable, conclusion is exact and certain, specificity and reappearation is better, and can be used for determination of Shuangshan Granules.

参考文献/References:

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备注/Memo

备注/Memo:
【收稿日期】2013-12-07 【作者简介】许子竞（1976－）男，广西金秀圣堂药业有限责任公司总工，博士，工程师，研究方向为中药制剂与质量标准
更新日期/Last Update: 1900-01-01