[1]卓少元 张保静.TCTE3 mRNA 在人肝癌细胞株中的表达特征[J].大众科技,2013,15(11):105.
点击复制

TCTE3 mRNA 在人肝癌细胞株中的表达特征
分享到:

《大众科技》[ISSN:1008-1151/CN:45-1235/N]

卷:
第15卷
期数:
2013年11期
页码:
105
栏目:
医药与卫生
出版日期:
2013-11-30

文章信息/Info

Title:
Expression and significance of TCTE3 mRNA in liver cancer cells cultured
文章编号:
1008-1151(2013)11-0105-03
作者:
卓少元 1 张保静 2
(1.广西中医药大学基础医学院,广西 南宁 530001; 2.广西中医药大学附属瑞康医院,广西 南宁 530011)
关键词:
TCTE3肝癌细胞缺氧
Keywords:
TCTE3 liver cancer cells hypoxia
分类号:
R36
文献标志码:
A
摘要:
探讨 TCTE3 mRNA 在人肝癌细胞株中的表达水平及意义。采用实时荧光定量 PCR 技术分别检测 TCTE3 基因在 常规培养或缺氧诱导(CoCl2 为诱导剂)的人肝癌细胞株中的表达情况。TCTE3 mRNA 在常规培养的人正常肝细胞株 L02,肝 癌细胞株 Bel-7402、SMMC-7721、HepG2 和 QGY-7701 中不表达或极低表达;但经缺氧诱导后,SMMC-7721 细胞中的 TCTE3 mRNA 表达量逐渐升高,4h 达到最大,随后逐渐降低,与 HIF1a mRNA 表达的曲线变化有一定相似之处,只是这种变化的时间 要早于 HIF1a 基因。在缺氧状态下肝癌细胞 TCTE3 mRNA 表达增加,且这种上调表达可能对 HIF1a mRNA 的表达有一定的促 进作用
Abstract:
To investigate genes expression and significance of TCTE3 mRNA in liver cancer cells cultured. TCTE3 mRNA were examined in liver cancer cells under conventional culture or hypoxia-induced culture by CoCl2 by real-time quantitative Polymerase chain reaction (PCR). No expression or low expression of TCTE3 mRNA levels in normal human liver L02 cells, and liver cancer Bel-7402, SMMC-7721, HepG2 or QGY-7701 cells. However, After SMMC-7721 cells were incubated with hypoxia-inducible CoCl2, the expression of TCTE3 mRNA began to increase gradually, peaked at 4h, and then decreased, which has some similarities with HIF1a mRNA expression curve, but such changes earlier than HIF1a mRNA. The expression of TCTE3 mRNA levels increased, which could promote HIF1a mRNA expression.

参考文献/References:

[1] 卓少元,方肇勤,卢文丽,等.邪毒壅盛证 H22 荷瘤小鼠肿瘤 组织基因的表达特征研究[J].时珍国医国药,2009,20(1): 31-33. [2] Rappold GA, Trowsdale J, Lichter P. Assignment of the human homologue of the mouse t-complex gene TCTE3 to human chromosome 6q27[J]. Genomics .1992,13(4):1337- 1339. [3] Rashid S,Grzmil P, Drenckhahn JD, et al . Disruption of the murine dynein light chain gene Tcte3-3 results in asthenozoospermia[J]. Reproduction .2010,139(1): 99-111. [4] Neesen J,Drenckhahn JD, Tiede S, et al . Identification of the human ortholog of the t-complex-encoded protein TCTE3 and evaluation as a candidate gene for primary ciliary dyskinesia[J]. Cytogenet Genome Res .2002,98(1): 38-44. [5] Brown JM. Tumor microenvironment and the response to anticancer therapy[J]. Cancer Biol Ther .2002,1(5):453-458. [6] Semenza GL. Targeting HIF-1 for cancer therapy[J]. Nat Rev Cancer .2003,3(10):721-732. [7] Burroughs SK,Kaluz S,Wang D,et al.Hypoxia inducible factor pathway inhibitors as anticancer therapeutics[J]. Future Med Chem . 2013, 5(5): 553-572. [8] Huang LE,Gu J,Schau M,et al. Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin- proteasome pathway[J]. Proc Natl Acad Sci U S A . 1998, 95(14):7987-7992. [9] 傅志超,王凤玫,程惠华.低氧培养的肝癌细胞中低氧诱导 因子 1 的表达与血管生成和细胞凋亡相关蛋白的关系[J]. 第二军医大学学报,2006,27(12):1328-1332. [10] Hubert A,Paris S,Piret JP,Ninane N,Raes M, Michiels C. Casein kinase 2 inhibition decreases hypoxia-inducible factor-1 activity under hypoxia through elevated p53 protein level[J]. J Cell Sci .2006, 119(Pt 16):3351-3362. [11] Bai X, Chan ED, Xu X. The protein of a new gene, Tctex4, interacts with protein kinase CK2beta subunit and is highly expressed in mouse testis[J]. Biochem Biophys Res Commun . 2003,307(1):86-91.

备注/Memo

备注/Memo:
【收稿日期】2013-10-16 【基金项目】广西卫生厅中医药科技专项(No.GZKZ10-53);广西教育厅科研项目(No.201010LX262)。 【作者简介】卓少元(1980-),男,河南人,广西中医药大学基础医学院副教授,博士学位,从事肝癌的中医药防治研究
更新日期/Last Update: 1900-01-01